Full Product Name
Dpf3 antibody - N-terminal region
Product Gene Name
anti-DPF3 antibody
[Similar Products]
Product Synonym Gene Name
2810403B03Rik; C78788; CERD4; cer-d4; BAF45C; 6530402L11Rik[Similar Products]
Antibody/Peptide Pairs
Dpf3 peptide (MBS3229322) is used for blocking the activity of Dpf3 antibody (MBS3204355)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
Synthetic peptide located within the following region: IHNPLKALGD QFYKEAIEHC RSYNSRLCAE RSVRLPFLDS QTGVAQNNCY
3D Structure
ModBase 3D Structure for P58269
Species Reactivity
Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Purity/Purification
Affinity Purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Homology
Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 86%
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-DPF3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-DPF3 antibody
This is a rabbit polyclonal antibody against Dpf3. It was validated on Western Blot
Target Description: Dpf3 is a muscle-specific component of the BAF complex, a multiprotein complex involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Dpf3 specifically binds acetylated lysines on histone 3 and 4 (H3K14ac, H3K9ac, H4K5ac, H4K8ac, H4K12ac, H4K16ac). In the complex, it acts as a tissue-specific anchor between histone acetylations and methylations and chromatin remodeling. It thereby probably plays an essential role in heart and skeletal muscle development. Belongs to the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their ***** state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
Product Categories/Family for anti-DPF3 antibody
Polyclonal; Transcription Factor; Transcription Regulation; Transcription Regulation; Cell Biology; Developmental Biology; Disease Related; Transcription Factors;
Applications Tested/Suitable for anti-DPF3 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Immunohistochemistry (IHC) of anti-DPF3 antibody
Rabbit Anti-Dpf3 Antibody
Formalin Fixed Paraffin Embedded Tissue: Human Adult heart
Observed Staining: Nuclear (not in cardiomyocytes but in fibrocytes in endomysium
Primary Antibody Concentration: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy2/3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec
Protocol located in Reviews and Data.
Western Blot (WB) of anti-DPF3 antibody
WB Suggested Anti-Dpf3 Antibody
Titration: 1.0 ug/ml
Positive Control: Mouse Spleen
NCBI/Uniprot data below describe general gene information for DPF3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_478119
[Other Products]
NCBI GenBank Nucleotide #
NM_058212
[Other Products]
UniProt Primary Accession #
P58269
[Other Products]
UniProt Related Accession #
P58269[Other Products]
NCBI Official Full Name
zinc finger protein DPF3 isoform 3
NCBI Official Synonym Full Names
D4, zinc and double PHD fingers, family 3
NCBI Official Symbol
Dpf3 [Similar Products]
NCBI Official Synonym Symbols
CERD4; BAF45C; C78788; cer-d4; 2810403B03Rik; 6530402L11Rik
[Similar Products]
NCBI Protein Information
zinc finger protein DPF3
UniProt Protein Name
Zinc finger protein DPF3
UniProt Synonym Protein Names
BRG1-associated factor 45C; BAF45C; Zinc finger protein cer-d4
Protein Family
Zinc finger protein
UniProt Gene Name
Dpf3 [Similar Products]
UniProt Synonym Gene Names
Baf45c; Cerd4; BAF45C [Similar Products]
UniProt Entry Name
DPF3_MOUSE
Research Articles on DPF3
1. DPF3 adds a further layer of complexity to the BAF complex by representing a tissue-specific anchor between histone acetylations as well as methylations and chromatin remodeling.
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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