Full Product Name
Mouse anti-Human CD25, PE Conjugated mAb
Product Gene Name
anti-CD25 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P25094
Form/Format
Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide
Experimental Methods
1.Take 100mul peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
2.Add 10mul labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light;
3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×);
4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5.Add 2 ml PBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant;
6.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 degree C then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3]
[Cell fixation: 2% formaldehyde solution]
Preparation and Storage
Store at 4 degree C. DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.
Other Notes
Small volumes of anti-CD25 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CD25 antibody
4A7G7B6 reacts with CD25 antigen, a chain of low-affinity interleukin-2 receptor (IL-2Ra), which is expressed on activated cells including T, B, NK cells and monocytes. The antigen also prsent on subset of thymocytes, HTLV-1 transformed T cell lines, EBV transformed B cells, myeloid precursors and oligodendrocytes. The high affinity IL-2 receptor is formed by the noncovalent association of of a (55 kDa, CD25), b (75 kDa, CD122), and g subunit (70 kDa, CD132). The interaction of IL-2 with IL-2R induces the activation and proliferation of T, B, NK cells and macrophages. CD4+/CD25+ cells might directly regulate the function of responsive T cells.
Applications Tested/Suitable for anti-CD25 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF)
NCBI/Uniprot data below describe general gene information for CD25. It may not necessarily be applicable to this product.
NCBI Accession #
CAA44297.1
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UniProt Primary Accession #
P25094
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Molecular Weight
17,946 Da
NCBI Official Full Name
CD25
UniProt Protein Name
Peripheral myelin protein 22
UniProt Synonym Protein Names
Protein CD25; SR13 myelin protein; Schwann cell membrane glycoprotein; SAG
UniProt Gene Name
Pmp22 [Similar Products]
UniProt Synonym Gene Names
Cd25; Pmp-22; PMP-22; SAG [Similar Products]
UniProt Comments for CD25
Might be involved in growth regulation, and in myelinization in the peripheral nervous system.
Product References and Citations for anti-CD25 antibody
1. Kishimoto, T. et al. (1998). Leucocyte Typing VI: White Cell Differentiation Antigens. Garland Publishing, Inc. London. 2. Robb RJ. et al. (1984). J. Exp. Med 160:1126. 3. Greene WC and Leonard WJ et al. (1986). Annu. Rev. Immunol. 4:69. 4. Ng WFet al. (2001). Leukemia 98: 2736.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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