PINK1, Polyclonal Antibody

BRPK; PARK6

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

The antibody detects endogenous levels of total PINK1 protein.

Antigen Affinity Purification

Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

1 mg/ml (lot specific)

Store at -20 degree C

Small volumes of anti-PINK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This gene encodes a serine/threonine protein kinase that localizes to mitochondria. It is thought to protect cells from stress-induced mitochondrial dysfunction. Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease.

Total protein Ab

Western Blot (WB), Immunohistochemistry (IHC)

Western Blot: 1:500-1:2000
Immunohistochemistry: 1:25-1:100

Gel: 10%SDS-PAGE Lysate: 40 μ g Lane: Human fetal brain tissue Primary antibody: 1/125 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds

30,104 Da

PTEN induced putative kinase 1

serine/threonine-protein kinase PINK1, mitochondrial

Serine/threonine-protein kinase PINK1, mitochondrial

PINK1_HUMAN

PINK1: Protects against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). It is necessary for PARK2 recruitment to dysfunctional mitochondria to initiate their degradation. Interats with PARK2. Highly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.11.1; Kinase, protein; Membrane protein, integral; Mitochondrial; NKF2 family; Other group; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: axon; chromatin; cytoplasm; cytoskeleton; cytosol; integral to mitochondrial outer membrane; membrane; mitochondrial inner membrane; mitochondrial intermembrane space; mitochondrial outer membrane; mitochondrial outer membrane translocase complex; mitochondrion; nucleus; perinuclear region of cytoplasm; TORC2 complex; ubiquitin ligase complex

Molecular Function: ATP binding; kinase activity; magnesium ion binding; protease activator activity; protease binding; protein binding; protein kinase activity; protein kinase B binding; protein serine/threonine kinase activity; ubiquitin protein ligase binding

Biological Process: activation of protein kinase B; macroautophagy; mitochondrion degradation; mitochondrion organization and biogenesis; negative regulation of JNK cascade; negative regulation of macroautophagy; negative regulation of neuron apoptosis; peptidyl-serine phosphorylation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of macroautophagy; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein amino acid phosphorylation; positive regulation of protein kinase B signaling cascade; positive regulation of protein ubiquitination; positive regulation of ubiquitin-protein ligase activity; protein amino acid phosphorylation; protein stabilization; protein ubiquitination; regulation of mitochondrial membrane potential; regulation of oxidative phosphorylation; regulation of protein complex assembly; regulation of protein ubiquitination; response to oxidative stress; response to stress; ubiquitin-dependent protein catabolic process

Disease: Parkinson Disease 6, Autosomal Recessive Early-onset

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