Product Name
DNASE1, siRNA
Full Product Name
DNASE1 siRNA (Mouse)
Product Synonym Names
DNL1; Deoxyribonuclease-1; Deoxyribonuclease I; DNase I
Product Gene Name
DNASE1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P49183
Specificity
DNASE1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse DNASE1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of DNASE1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
DNASE1 sirna
siRNA to inhibit DNASE1 expression using RNA interference
Applications Tested/Suitable for DNASE1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for DNASE1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_034191.3
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NCBI GenBank Nucleotide #
NM_010061.5
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UniProt Primary Accession #
P49183
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UniProt Secondary Accession #
O70532[Other Products]
UniProt Related Accession #
P49183[Other Products]
Molecular Weight
32,027 Da
NCBI Official Full Name
deoxyribonuclease-1
NCBI Official Synonym Full Names
deoxyribonuclease I
NCBI Official Symbol
Dnase1 [Similar Products]
NCBI Official Synonym Symbols
Dnl1; DNaseI; AI788650
[Similar Products]
NCBI Protein Information
deoxyribonuclease-1
UniProt Protein Name
Deoxyribonuclease-1
UniProt Synonym Protein Names
Deoxyribonuclease I; DNase I
Protein Family
Deoxyribonuclease
UniProt Gene Name
Dnase1 [Similar Products]
UniProt Synonym Gene Names
Dnl1; DNase I [Similar Products]
UniProt Entry Name
DNAS1_MOUSE
UniProt Comments for DNASE1
DNASE1: Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization. Defects in DNASE1 are a cause of susceptibility to systemic lupus erythematosus (SLE). A chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Belongs to the DNase I family.
Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; Deoxyribonuclease; EC 3.1.21.1
Cellular Component: extracellular region; nucleus
Molecular Function: endodeoxyribonuclease activity, producing 5'-phosphomonoesters; nuclease activity; hydrolase activity; endonuclease activity; deoxyribonuclease activity; deoxyribonuclease I activity; actin binding; endodeoxyribonuclease activity
Biological Process: apoptosis; DNA catabolic process; DNA catabolic process, endonucleolytic
Research Articles on DNASE1
1. HMGN1 binds to CpG island-containing promoters and affects the organization of nucleosomes, DNase I hypersensitivity, and the transcriptional profile of mouse embryonic stem cells and neural progenitors.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
