ALAS-H, Polyclonal Antibody

ALAS3; ALASH; 5-aminolevulinate synthase, nonspecific, mitochondrial; ALAS-H; 5-aminolevulinic acid synthase 1; Delta-ALA synthase 1; Delta-aminolevulinate synthase 1

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Recognizes endogenous levels of ALAS-H protein.

The antibody was purified by immunogen affinity chromatography.

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Small volumes of anti-ALAS1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Rabbit polyclonal antibody to ALAS-H

Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

WB: 1/500 - 1/2000; IF/ICC: 1/10 - 1/100

Western blot analysis of ALAS-H expression in SW620 (A), HepG2 (B), mouse liver (C) whole cell lysates.

Immunofluorescent analysis of ALAS-H staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

12,961 Da

5'-aminolevulinate synthase 1

5-aminolevulinate synthase, nonspecific, mitochondrial

5-aminolevulinate synthase, nonspecific, mitochondrial

ALAS3; ALASH; ALAS-H  [Similar Products]

HEM1_HUMAN

This gene encodes the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. The enzyme encoded by this gene is the housekeeping enzyme; a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate. A pseudogene of this gene is located on chromosome 12. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2015]

ALAS1: the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. The enzyme encoded by this gene is the housekeeping enzyme; a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate. A pseudogene of this gene is located on chromosome 12. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Dec 2011]

Protein type: Amino Acid Metabolism - glycine, serine and threonine; EC 2.3.1.37; Transferase; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; Mitochondrial

Chromosomal Location of Human Ortholog: 3p21.1

Cellular Component: cytoplasm; mitochondrial matrix; mitochondrion; nucleoplasm

Molecular Function: 5-aminolevulinate synthase activity; protein binding; pyridoxal phosphate binding

Biological Process: heme biosynthetic process; mitochondrion organization and biogenesis; protoporphyrinogen IX biosynthetic process

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