BIGH3 (Fasciclin domain 4), Recombinant Protein

TGFBI; BIGH3; BIGH3; Fasciclin domain 4; Beta ig-h3; Kerato-epithelin; RGD-containing collagen-associated protein; RGD-CAP; Transforming growth factor-beta-induced protein ig-h3; RGD containing collagen associated protein; Beta ig h3; AI747162; big h3; CSD3; Kerato epithelin; LCD1; MGC150270; RGD CAP; TGFBI transforming growth factor; beta induced; 68kDa; AI181842; Beta ig; CDB1; CDG2; CDGG1; CSD; CSD1; CSD2; EBMD; Transforming growth factor beta induced protein ig h3.

For Research Use Only. Not for use in diagnostic procedures.

E Coli

> 95% by SDS-PAGE

Liquid. 20mM Tris pH8.0

1 mg/ml (determined by Bradford assay) (lot specific)

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Small volumes of BIGH3 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

BIGH3, also known as TGFBI and betaig-h3, is an extracellular matrix protein induced by transforming growth factor (TGF)-beta 1. BIGH3 protein is involved in cell growth, cell differentiation, wound healing and cell adhesion. In addition, some missense mutations of BIGH3 were identified in families affected with human autosomal dominant corneal dystrophies. BIGH3 gene encodes for a 683 amino-acid protein containing an RGD motif and four internal repeated domains which have highly conserved sequences founded in several species (Fasciclin domain). Recombinant human BIGH3 protein (fourth FAS domain) was expressed in E. coli and purified by using conventional chromatography techniques.

Cell Cycle

SDS-PAGE

14.5 kDa (135 aa), confirmed by MALDI-TOF.

transforming growth factor beta induced

transforming growth factor-beta-induced protein ig-h3

Transforming growth factor-beta-induced protein ig-h3

BIGH3; Beta ig-h3; RGD-CAP  [Similar Products]

This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in this gene are associated with multiple types of corneal dystrophy. [provided by RefSeq, Jul 2008]

Plays a role in cell adhesion (PubMed:8024701). May play a role in cell-collagen interactions ().

Billings PC, et al. (2002). J Biol Chem. 277. 28003-28009; Munier FL. et al. (2002). Invest Ophthalmol Vis Sci. 43.949-954.; Jung-Eun Kim, et al. (2000) J Biol Chem. 275. 30907-30915;

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