Product Name
WFS1, Polyclonal Antibody
Popular Item
Full Product Name
WFS1 Antibody
Product Synonym Names
DFNA14; DFNA38; DFNA6; DIDMOAD; WFRS; WFS; Wolfram syndrome 1 (wolframin); Wolfram syndrome; WOLFRAMIN
Product Gene Name
anti-WFS1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O76024
Species Reactivity
Human, Mouse, Rat
Specificity
WFS1 antibody detects endogenous levels of total WFS1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
A synthesized peptide derived from human WFS1
Subcellular Location
Endoplasmic Reticulum; Endoplasmic Reticulum Membrane; Multipass Membrane Protein
Tissue Specificity
Highly expressed in heart followed by brain, placenta, lung and pancreas. Weakly expressed in liver, kidney and skeletal muscle. Also expressed in islet and beta-cell insulinoma cell line.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (92%), Bovine (100%), Horse (100%), Sheep (92%), Rabbit (80%), Dog (92%), Chicken (91%)
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Other Notes
Small volumes of anti-WFS1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-WFS1 antibody
Description: Wolfram syndrome protein (WFS1) is an 890 amino acid protein that contains a cytoplasmic N-terminal domain, followed by nine-transmembrane domains and a luminal C-terminal domain. WFS1 is predominantly localized to the endoplasmic reticulum (ER) (1) and its expression is induced in response to ER stress, partially through transcriptional activation (2, 3). Research studies have shown that mutations in the WFS1 gene lead to Wolfram syndrome, an autosomal recessive neurodegenerative disorder defined by young-onset, non-immune, insulin-dependent diabetes mellitus and progressive optic atrophy (4).
Function: Participates in the regulation of cellular Ca2+ homeostasis, at least partly, by modulating the filling state of the endoplasmic reticulum Ca2+ store.
Applications Tested/Suitable for anti-WFS1 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-WFS1 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
Immunohistochemistry (IHC) of anti-WFS1 antibody
MBS9607222 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

Western Blot (WB) of anti-WFS1 antibody
Western blot analysis of extracts of mouse brain, using WFS1 antibody. The lane on the left is treated with the antigen-specific peptide.

NCBI/Uniprot data below describe general gene information for WFS1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001139325.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001145853.1
[Other Products]
UniProt Primary Accession #
O76024
[Other Products]
UniProt Secondary Accession #
Q8N6I3; Q9UNW6; B2R797; D3DVT1[Other Products]
UniProt Related Accession #
O76024[Other Products]
Molecular Weight
Observed: 100 kDa
Predicted: 101 kDa
NCBI Official Full Name
wolframin
NCBI Official Synonym Full Names
wolframin ER transmembrane glycoprotein
NCBI Official Symbol
WFS1 [Similar Products]
NCBI Official Synonym Symbols
WFS; WFRS; WFSL; CTRCT41
[Similar Products]
NCBI Protein Information
wolframin
UniProt Protein Name
Wolframin
UniProt Gene Name
WFS1 [Similar Products]
NCBI Summary for WFS1
This gene encodes a transmembrane protein, which is located primarily in the endoplasmic reticulum and ubiquitously expressed with highest levels in brain, pancreas, heart, and insulinoma beta-cell lines. Mutations in this gene are associated with Wolfram syndrome, also called DIDMOAD (Diabetes Insipidus, Diabetes Mellitus, Optic Atrophy, and Deafness), an autosomal recessive disorder. The disease affects the brain and central nervous system. Mutations in this gene can also cause autosomal dominant deafness 6 (DFNA6), also known as DFNA14 or DFNA38. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2009]
UniProt Comments for WFS1
Participates in the regulation of cellular Ca2+ homeostasis, at least partly, by modulating the filling state of the endoplasmic reticulum Ca2+ store.
Research Articles on WFS1
1. Altered expression of WFS1 and NOTCH2 genes may play a role in pathogenesis and development of DN in patients with T2DM.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.