MKS1, Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab); Meckel syndrome type 1 protein; MKS1

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Ig

Rabbit

This MKS1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 90-124 amino acids from the human region of human MKS1.

Purified through a protein A column, followed by peptide affinity purification.

Supplied in PBS with 0.09% (W/V) sodium azide.

Maintain refrigerated at 2-8 degree C for up to 2 weeks.
For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-MKS1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. Involved in centrosome migration to the apical cell surface during early ciliogenesis. Required for ciliary structure and function, including a role in regulating length and appropriate number through modulating centrosome duplication. Required for cell branching morphology.

Primary Antibodies

Immunofluorescence (IF), Flow Cytometry (FC/FACS), Western Blot (WB), ELISA (EIA)

IF: 1:25
FC: 1:25
WB: 1:2000

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Pdx1 with AP22330a at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HepG2 cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).

Overlay histogram showing HepG2 cells stained with AP22330a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22330a, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

All lanes : Anti-MKS1 Antibody (N-Term) at 1:2000 dilution
Lane 1: Human kidney lysate
Lane 2: Human lung lysate


Lysates/proteins at 20 ug per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 65 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

64528

Meckel syndrome, type 1

Meckel syndrome type 1 protein

Meckel syndrome type 1 protein

The protein encoded by this gene localizes to the basal body and is required for formation of the primary cilium in ciliated epithelial cells. Mutations in this gene result in Meckel syndrome type 1 and in Bardet-Biedl syndrome type 13. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. Involved in centrosome migration to the apical cell surface during early ciliogenesis. Required for ciliary structure and function, including a role in regulating length and appropriate number through modulating centrosome duplication. Required for cell branching morphology.

Kyttaelae M.,et al.Nat. Genet. 38:155-157(2006). Ota T.,et al.Nat. Genet. 36:40-45(2004). Zody M.C.,et al.Nature 440:1045-1049(2006). Ebert L.,et al. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases. Dawe H.R.,et al.Hum. Mol. Genet. 16:173-186(2007).

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