Product Name
ADAMTS17, siRNA
Full Product Name
ADAMTS17 siRNA (Human)
Product Synonym Names
A disintegrin and metalloproteinase with thrombospondin motifs 17; ADAM-TS 17; ADAM-TS17; ADAMTS-17
Product Gene Name
ADAMTS17 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8TE56
Specificity
ADAMTS17 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ADAMTS17 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ADAMTS17 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ADAMTS17 sirna
siRNA to inhibit ADAMTS17 expression using RNA interference
Applications Tested/Suitable for ADAMTS17 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ADAMTS17. It may not necessarily be applicable to this product.
NCBI Accession #
NP_620688.2
[Other Products]
NCBI GenBank Nucleotide #
NM_139057.2
[Other Products]
UniProt Primary Accession #
Q8TE56
[Other Products]
UniProt Secondary Accession #
Q2I7G4; Q6ZN75[Other Products]
UniProt Related Accession #
Q8TE56[Other Products]
Molecular Weight
54,757 Da
NCBI Official Full Name
A disintegrin and metalloproteinase with thrombospondin motifs 17 preproprotein
NCBI Official Synonym Full Names
ADAM metallopeptidase with thrombospondin type 1 motif, 17
NCBI Official Symbol
ADAMTS17 [Similar Products]
NCBI Protein Information
A disintegrin and metalloproteinase with thrombospondin motifs 17
UniProt Protein Name
A disintegrin and metalloproteinase with thrombospondin motifs 17
Protein Family
A disintegrin and metalloproteinase with thrombospondin motifs
UniProt Gene Name
ADAMTS17 [Similar Products]
UniProt Synonym Gene Names
ADAM-TS 17; ADAM-TS17; ADAMTS-17 [Similar Products]
UniProt Entry Name
ATS17_HUMAN
NCBI Summary for ADAMTS17
This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. ADAMTS family members share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The protein encoded by this gene has a high sequence similarity to the protein encoded by ADAMTS19, another family member. The function of this protein has not been determined. [provided by RefSeq, Jul 2008]
UniProt Comments for ADAMTS17
ADAMTS17: Defects in ADAMTS17 are the cause of Weill-Marchesani- like syndrome (WMLS). It is a disorder characterized by many of the key features of Weill-Marchesani syndrome, including lenticular myopia, ectopia lentis, glaucoma, spherophakia and short stature. However, the characteristic brachydactyly or decreased joint flexibility of Weill-Marchesani syndrome are absent. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Protease; Secreted, signal peptide; Secreted; EC 3.4.24.-
Chromosomal Location of Human Ortholog: 15q24
Cellular Component: proteinaceous extracellular matrix
Molecular Function: zinc ion binding; metalloendopeptidase activity
Biological Process: proteolysis
Disease: Weill-marchesani-like Syndrome
Research Articles on ADAMTS17
1. higher Adamts17 expression is found in several human cancer cell subtypes, especially in breast ductal carcinoma and there is an inverse correlation between higher Adamts17 expression and patients' survival.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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