TGF beta R2, Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab); AAT3; FAA3; LDS2; MFS2; RIIC; LDS1B; LDS2B; TAAD2; TGFR-2; TGFbeta-RII; TGF-beta receptor type-2; TGF-beta type II receptor; Transforming growth factor-beta receptor type II; TGF-beta receptor type II; TbetaR-II; TGFBR2

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol

Store at -20 ? for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ?.

Small volumes of anti-TGFBR2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Transmembrane serine/threonine kinase forming with the TGF-beta type I serine/threonine kinase receptor, TGFBR1, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFRB1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways.

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

WB~~1:100~1:500
IHC-P~~1:100~1:500

HepG2 cell lysates probed with Rabbit Anti-TGF beta R2 Polyclonal Antibody, Unconjugated (MBS9216787) at 1:300 overnight at 4?C. Followed by conjugation to secondary antibody at 1:500 for 90 min at 37°C.

Formalin-fixed and paraffin embedded: rabbit carotid artery labeled with Anti-TGF-beta-R2/TGFBR2 Polyclonal Antibody (MBS9216787), Unconjugated 1:600 followed by conjugation to the secondary antibody and DAB staining

64568

transforming growth factor beta receptor II

TGF-beta receptor type-2

TGF-beta receptor type-2

TGFR-2; TGF-beta receptor type II; TbetaR-II  [Similar Products]

TGFR2_HUMAN

This gene encodes a member of the Ser/Thr protein kinase family and the TGFB receptor subfamily. The encoded protein is a transmembrane protein that has a protein kinase domain, forms a heterodimeric complex with another receptor protein, and binds TGF-beta. This receptor/ligand complex phosphorylates proteins, which then enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation. Mutations in this gene have been associated with Marfan Syndrome, Loeys-Deitz Aortic Aneurysm Syndrome, and the development of various types of tumors. Alternatively spliced transcript variants encoding different isoforms have been characterized. [provided by RefSeq, Jul 2008]

TGFBR2: a TKL kinase of the serine/threonine-protein kinase receptor (STKR) family. R1 and R2 TGF-beta receptors dimerize after binding TGF-beta at the cell surface. Binds to DAXX. Defects can cause esophageal cancer.

Protein type: Kinase, protein; Membrane protein, integral; Protein kinase, Ser/Thr (receptor); Protein kinase, TKL; EC 2.7.11.30; Oncoprotein; TKL group; STKR family; Type2 subfamily

Chromosomal Location of Human Ortholog: 3p22

Cellular Component: plasma membrane; integral to membrane; caveola; cytosol; receptor complex; external side of plasma membrane

Molecular Function: transforming growth factor beta receptor activity; protein binding; glycosaminoglycan binding; transforming growth factor beta receptor activity, type II; transforming growth factor beta binding; metal ion binding; mitogen-activated protein kinase kinase kinase binding; SMAD binding; transmembrane receptor protein serine/threonine kinase activity; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: lens development in camera-type eye; wound healing; apoptosis; positive regulation of smooth muscle cell proliferation; heart development; myeloid dendritic cell differentiation; gastrulation; palate development; positive regulation of tolerance induction to self antigen; protein amino acid phosphorylation; negative regulation of cardiac muscle cell proliferation; transforming growth factor beta receptor signaling pathway; positive regulation of mesenchymal cell proliferation; positive regulation of cell proliferation; response to glucose stimulus; positive regulation of NK T cell differentiation; vasculogenesis; response to nutrient; positive regulation of T cell tolerance induction; aging; response to drug; blood vessel development; receptor-mediated endocytosis; Notch signaling pathway; smoothened signaling pathway; organ regeneration; positive regulation of skeletal muscle regeneration; in utero embryonic development; embryonic hemopoiesis; common-partner SMAD protein phosphorylation; peptidyl-threonine phosphorylation; embryonic cranial skeleton morphogenesis; regulation of cell proliferation; patterning of blood vessels; peptidyl-serine phosphorylation; positive regulation of angiogenesis; gut development; regulation of gene expression; response to mechanical stimulus; response to estrogen stimulus; positive regulation of B cell tolerance induction; activation of protein kinase activity; brain development; negative regulation of transforming growth factor beta receptor signaling pathway; embryo implantation

Disease: Colorectal Cancer, Hereditary Nonpolyposis, Type 6; Loeys-dietz Syndrome 2; Esophageal Cancer

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