Full Product Name
LEPR siRNA (Rat)
Product Synonym Names
FA; OBR; Leptin receptor; LEP-R; OB receptor; OB-R; CD antigen CD295
Product Gene Name
LEPR sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q62959
Specificity
LEPR siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat LEPR gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of LEPR sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
LEPR sirna
siRNA to inhibit LEPR expression using RNA interference
Applications Tested/Suitable for LEPR sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for LEPR. It may not necessarily be applicable to this product.
NCBI Accession #
NP_036728.1
[Other Products]
NCBI GenBank Nucleotide #
NM_012596.1
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UniProt Primary Accession #
Q62959
[Other Products]
UniProt Secondary Accession #
O35772; O35773; O54805; P70493; P70494; P70495; P97589; Q62960; Q63007; Q63385; Q63386[Other Products]
UniProt Related Accession #
Q62959[Other Products]
Molecular Weight
101,395 Da
NCBI Official Full Name
leptin receptor
NCBI Official Synonym Full Names
leptin receptor
NCBI Official Symbol
Lepr [Similar Products]
NCBI Official Synonym Symbols
Fa
[Similar Products]
NCBI Protein Information
leptin receptor
UniProt Protein Name
Leptin receptor
UniProt Synonym Protein Names
OB receptor; OB-R; CD_antigen: CD295
Protein Family
Leptin receptor
UniProt Gene Name
Lepr [Similar Products]
UniProt Synonym Gene Names
Fa; Obr; LEP-R; OB-R [Similar Products]
UniProt Entry Name
LEPR_RAT
NCBI Summary for LEPR
receptor for leptin; may have a role in body weight maintainence [RGD, Feb 2006]
UniProt Comments for LEPR
LEPR: the receptor for leptin (obesity factor). A single-transmembrane-domain receptor of the cytokine receptor family. On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Five alternatively spliced transcript variants have been reported.
Protein type: Membrane protein, integral; Receptor, cytokine
Research Articles on LEPR
1. Hipocampal lepr expression was lower in experimental diabetic group than control group. learning performance of the control group was found to be better than the diabetic group. Lepr gene may be related to learning performance in diabetic rats.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
