Lamin A and C, Monoclonal Antibody

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG1

131C3

Mouse

131C3 reacts with an epitope loCated between residues 319-566 in lamin A and C.

Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

Store at 4 degree C, or in small aliquots at -20 degree C.

Small volumes of anti-lmna antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Recent evidence has revealed that mutations in A-type lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A-type lamins coincides with cell differentiation and as A-type lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A-type lamins.

Nuclear proteins, Cytoskeleton

Flow Cytometry (FC), Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Western Blot (WB)

131C3 is suitable for immunocytochemistry, immunohistochemistry on frozen sections, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titRation; recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting appliCations.

Figure 1: Immunoblotting of 131C3 recognizing Nuclear Lamins A and C in human fibroblast lysate

Figure 2: Immunohistochemistry on frozen tissue section of human colon showing nuclear lamina staining in epithelial and connective tissue cells

74,919 Da[Similar Products]

lamin A/C<

lamin-A

Lamin-A

LMNA_XENLA

Function: Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.

Subcellular location: Nucleus.

Miscellaneous: There are at least five different lamins in Xenopus: the somatic lamins L(I), L(II), and A; the oocyte germinal vesicle lamin L(III); and the male germ cells lamin l(IV).

Sequence similarities: Belongs to the intermediate filament family.

Pugh, G. E., Coates, P. J., Lane, E. B., Raymond, Y., and Quinlan, R. A. (1997). Distinct nuclear assembly pathways for lamins A and C lead to their increase during quiescence in Swiss 3T3 cells, J Cell Sci 110, 2483-93. Neri, L. M., Raymond, Y., Giordano, A., Borgatti, P., Marchisio, M., Capitani, S., and Martelli, A. M. (1999). Spatial distribution of lamin A and B1 in the K562 cell nuclear matrix stabilized with metal ions, J Cell Biochem 75, 36-45. Neri, L. M., Raymond, Y., Giordano, A., Capitani, S., and Martelli, A. M. (1999). Lamin A is part of the internal nucleoskeleton of Human erythroleukemia cells, J Cell Physiol 178, 284-95.

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