Bi-Phospho-FGFR (Y653/654), Polyclonal Antibody

Fibroblast growth factor receptor 1; FGFR-1; Basic fibroblast growth factor receptor 1; BFGFR; bFGF-R-1; Fms-like tyrosine kinase 2; FLT-2; N-sam; Proto-oncogene c-Fgr; CD331; FGFR1; BFGFR; CEK; FGFBR; FLG; FLT2; HBGFR

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This FGFR Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y653/654 of human FGFR.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.49 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-FGFR1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds both acidic and basic fibroblast growth factors and is involved in limb induction. Mutations in this gene can lead to Pfeiffer syndrome and Jackson-Weiss syndrome. The genomic organization of this gene is very similar to family members 2-4, encompassing 19 exons that are subject to complex alternative splicing, which allows for structural, tissue expression and ligand affinity variations among the isoforms.

Cancer; Cardiovascular; Neuroscience; Signal Transduction; Phospho Antibodies

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)

WB~~1:1000

Western blot analysis of FGFR (arrow) using rabbit polyclonal Phospho-FGFR-pY653/4. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FGFR gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

91868

fibroblast growth factor receptor 1

fibroblast growth factor receptor 1

Fibroblast growth factor receptor 1

BFGFR; CEK; FGFBR; FLG; FLT2; HBGFR; FGFR-1; BFGFR; bFGF-R-1; FLT-2  [Similar Products]

FGFR1_HUMAN

Hu, Y., et al., J. Biol. Chem. 279(28):29325-29335 (2004). Ibrahimi, O.A., et al., Biochemistry 43(16):4724-4730 (2004). Sato, N., et al., J. Clin. Endocrinol. Metab. 89(3):1079-1088 (2004). Lundin, L., et al., Exp. Cell Res. 287(1):190-198 (2003). Jiao, J., et al., Arch. Biochem. Biophys. 410(2):187-200 (2003).

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