Product Name
Amylase, alpha, Porcine Pancreas (AMY2), Enzyme
Full Product Name
Amylase, alpha, Porcine Pancreas
Product Gene Name
AMY2 enzyme
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 4; NC_010446.4 (126313702..126321854). Location: 4; 4
3D Structure
ModBase 3D Structure for P00690
Purity/Purification
Purified
Purified. Contaminants
Form/Format
Supplied as a lyophilized powder from 150mM sodium chloride, 3mM calcium chloride. Reconstitute with Tris saline buffer.
Activity
~230Units/mg powder @ 37 degree C
Specific Activity
~480Units/mg
Unit Definition
One unit will catalyze the hydrolysis of one micromole of 2-chloro-4-mitrophenyl-alpha-D-maltrtrioside to yield 2-chloro-4-nitrophenol per minute at 37 degree C.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile tris saline buffer. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of AMY2 enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
AMY2 enzyme
Alpha Amylase acts upon large polysaccharides at the alpha-1,4-glucoside bonds. The hydrolytic products have alpha configuration. The activity is present in all living organisms, however the enzymes vary remarkably even from tissue to tissue within a single species.
Product Categories/Family for AMY2 enzyme
Molecular Biology; MB-Enzymes, Glycosidase
NCBI/Uniprot data below describe general gene information for AMY2. It may not necessarily be applicable to this product.
NCBI Accession #
AAF02828.1
[Other Products]
UniProt Primary Accession #
P00690
[Other Products]
UniProt Secondary Accession #
Q9TUE4[Other Products]
UniProt Related Accession #
P00690[Other Products]
Molecular Weight
57,086 Da[Similar Products]
NCBI Official Full Name
alpha-amylase
NCBI Official Symbol
AMY2 [Similar Products]
NCBI Official Synonym Symbols
AMY2B
[Similar Products]
NCBI Protein Information
pancreatic alpha-amylase; PA; 1,4-alpha-D-glucan glucanohydrolase
UniProt Protein Name
Pancreatic alpha-amylase
UniProt Synonym Protein Names
1,4-alpha-D-glucan glucanohydrolase
Protein Family
Probable alpha-amylase
UniProt Gene Name
AMY2 [Similar Products]
UniProt Synonym Gene Names
PA [Similar Products]
UniProt Entry Name
AMYP_PIG
UniProt Comments for AMY2
Catalytic activity: Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.
Cofactor: Binds 1 calcium ion per subunit.Binds 1 chloride ion per subunit.
Subcellular location: Secreted › extracellular space.
Miscellaneous: The two forms of this enzyme, I and II, show very similar activities, molecular masses, and compositions and differ only in their isoelectric points. As no evidence for two variants were in the cDNA library of Ref.1, it is most likely that isoform I (PPAI) and isoform II (PPAII) are two forms of the same protein.
Sequence similarities: Belongs to the glycosyl hydrolase 13 family.
Research Articles on AMY2
1. x-ray crystallographic structure of the pig pancreatic alpha-amylase complexed with rho-nitrophenyl-alpha-D-maltoside-flexibility in the active site
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
