Product Name
Angiopoietin 4 (ANGPT4), Recombinant Protein
Full Product Name
Recombinant Angiopoietin 4 (ANGPT4)
Product Gene Name
ANGPT4 recombinant protein
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Matching Pairs
Unconjugated
Antibody: Angiopoietin 4 (ANGPT4) (MBS2006130)
Immunogen: Angiopoietin 4 (ANGPT4) (MBS2009897)
Matching Pairs
APC-CY7 Conjugated Antibody: Angiopoietin 4 (ANGPT4) (MBS2045749)
Immunogen: Angiopoietin 4 (ANGPT4) (MBS2009897)
Matching Pairs
PE Conjugated Antibody: Angiopoietin 4 (ANGPT4) (MBS2045750)
Immunogen: Angiopoietin 4 (ANGPT4) (MBS2009897)
Matching Pairs
APC Conjugated Antibody: Angiopoietin 4 (ANGPT4) (MBS2045751)
Immunogen: Angiopoietin 4 (ANGPT4) (MBS2009897)
Matching Pairs
Cy3 Conjugated Antibody: Angiopoietin 4 (ANGPT4) (MBS2045752)
Immunogen: Angiopoietin 4 (ANGPT4) (MBS2009897)
Matching Pairs
FITC Conjugated Antibody: Angiopoietin 4 (ANGPT4) (MBS2045753)
Immunogen: Angiopoietin 4 (ANGPT4) (MBS2009897)
Matching Pairs
HRP Conjugated Antibody: Angiopoietin 4 (ANGPT4) (MBS2045754)
Immunogen: Angiopoietin 4 (ANGPT4) (MBS2009897)
Matching Pairs
Biotin Conjugated Antibody: Angiopoietin 4 (ANGPT4) (MBS2092309)
Immunogen: Angiopoietin 4 (ANGPT4) (MBS2009897)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF - FLRQHYDAK PNGRDDRYCE SMMKERKLTS PCKDVNTFIH GTKKNIRAIC GKKGSPYGEN FRISNSPFQI TTCTHSGASP RPPCGYRAFK DFRYIVIACE DGWPVHFDES FISP
Chromosome Location
Chromosome: 14; NC_000080.6 (51763892..51773590, complement). Location: 14 C2; 14
3D Structure
ModBase 3D Structure for Q3TMQ6
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Organism
Mus musculus (Mouse)
Expression System
Prokaryotic expression
Residues
Phe32~Pro144 (Accession # Q3TMQ6) with two N-terminal Tags, His-tag and GST-tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customerâs specifications, please inquire.
Other Notes
Small volumes of ANGPT4 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ANGPT4 recombinant protein
About the Marker: Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
Applications Tested/Suitable for ANGPT4 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
SDS-Page of ANGPT4 recombinant protein
NCBI/Uniprot data below describe general gene information for ANGPT4. It may not necessarily be applicable to this product.
NCBI Accession #
NP_808212.2
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NCBI GenBank Nucleotide #
NM_177544.4
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UniProt Primary Accession #
Q3TMQ6
[Other Products]
UniProt Secondary Accession #
Q3UWE7; Q80XS4; Q80Z85[Other Products]
UniProt Related Accession #
Q3TMQ6[Other Products]
NCBI Official Full Name
angiogenin-4
NCBI Official Synonym Full Names
angiogenin, ribonuclease A family, member 4
NCBI Official Symbol
Ang4 [Similar Products]
NCBI Official Synonym Symbols
Raa4; Rnase5d
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NCBI Protein Information
angiogenin-4; ribonuclease A a4
UniProt Protein Name
Angiogenin-4
Protein Family
Angiopoietin
UniProt Gene Name
Ang4 [Similar Products]
UniProt Entry Name
ANG4_MOUSE
UniProt Comments for ANGPT4
ANG4: Has bactericidal activity against E.faecalis and L.monocytogenes, but not against L.innocua and E.coli. Promotes angiogenesis (in vitro). Has low ribonuclease activity (in vitro). Promotes proliferation of melanoma cells, but not of endothelial cells or fibroblasts (in vitro). Belongs to the pancreatic ribonuclease family.
Protein type: EC 3.1.27.-
Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region; basement membrane; cytoplasmic vesicle; nucleus; secretory granule
Molecular Function: nuclease activity; nucleic acid binding; hydrolase activity; endonuclease activity; ribonuclease activity
Biological Process: defense response to bacterium; RNA catabolic process; positive regulation of cell proliferation; antibacterial humoral response; angiogenesis
Research Articles on ANGPT4
1. The cellular source of mouse Ang4 in the colon following T. muris infection is the goblet cell and expression is under the control of IL-13.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.