Product Name
AF5q31 (AFF4), Polyclonal Antibody
Full Product Name
AF5q31 Antibody (N-term)
Product Synonym Names
AF4/FMR2 family member 4; ALL1-fused gene from chromosome 5q31 protein; Protein AF-5q31; Major CDK9 elongation factor-associated protein; AFF4; AF5Q31; MCEF
Product Gene Name
anti-AFF4 antibody
[Similar Products]
Antibody/Peptide Pairs
AF5q31 peptide (MBS9228345) is used for blocking the activity of AF5q31 antibody (MBS9202542)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Positions
1-30
3D Structure
ModBase 3D Structure for Q9UHB7
Species Reactivity
Human (Predicted Reactivity: Mouse)
Specificity
This AF5q31 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human AF5q31.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (lot specific)
Antigen Type
Synthetic Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-AFF4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-AFF4 antibody
Cell Biology
Applications Tested/Suitable for anti-AFF4 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-AFF4 antibody
WB~~1:1000
Western Blot (WB) of anti-AFF4 antibody
Western blot analysis of AF5q31 Antibody (N-term) in HL-60 cell line lysates (35ug/lane). AF5q31 (arrow) was detected using the purified Pab.
NCBI/Uniprot data below describe general gene information for AFF4. It may not necessarily be applicable to this product.
NCBI Accession #
NP_055238.1
[Other Products]
NCBI GenBank Nucleotide #
NM_014423.3
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UniProt Primary Accession #
Q9UHB7
[Other Products]
UniProt Secondary Accession #
Q498B2; Q59FB3; Q6P592; Q8TDR1; Q9P0E4; B2RP19; B7WPD2[Other Products]
UniProt Related Accession #
Q9UHB7[Other Products]
NCBI Official Full Name
AF4/FMR2 family member 4
NCBI Official Synonym Full Names
AF4/FMR2 family, member 4
NCBI Official Symbol
AFF4 [Similar Products]
NCBI Official Synonym Symbols
MCEF; CHOPS; AF5Q31
[Similar Products]
NCBI Protein Information
AF4/FMR2 family member 4
UniProt Protein Name
AF4/FMR2 family member 4
UniProt Synonym Protein Names
ALL1-fused gene from chromosome 5q31 protein; Protein AF-5q31; Major CDK9 elongation factor-associated protein
Protein Family
AF4/FMR2 family
UniProt Gene Name
AFF4 [Similar Products]
UniProt Synonym Gene Names
AF5Q31; MCEF; Protein AF-5q31 [Similar Products]
UniProt Entry Name
AFF4_HUMAN
NCBI Summary for AFF4
The protein encoded by this gene belongs to the AF4 family of transcription factors involved in leukemia. It is a component of the positive transcription elongation factor b (P-TEFb) complex. A chromosomal translocation involving this gene and MLL gene on chromosome 11 is found in infant acute lymphoblastic leukemia with ins(5;11)(q31;q31q23). [provided by RefSeq, Oct 2011]
UniProt Comments for AFF4
MCEF: a putative transcription factor. Component of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor b (P-TEFb). Genetic fusion of its gene with MLL has been observed in a subset of acute lymphoblastic leukemia patients. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Oncoprotein; Transcription factor
Chromosomal Location of Human Ortholog: 5q31
Cellular Component: nucleoplasm; transcription elongation factor complex; mitochondrion; nucleolus; nucleus
Molecular Function: protein binding; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; spermatid development
Disease: Chops Syndrome
Product References and Citations for anti-AFF4 antibody
Lin, C., et al. Mol. Cell 37(3):429-437(2010)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
Niedzielski, M.F., et al. Int. J. Biol. Sci. 3(4):225-236(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Estable, M.C., et al. J. Biomed. Sci. 9(3):234-245(2002)
Research Articles on AFF4
1. HIV-1 Tat recruits transcription elongation factors dispersed along a flexible AFF4 scaffold.
Precautions
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Disclaimer
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