NKX3-2, Polyclonal Antibody

NK3 homeobox 2; BAPX1, Homeobox protein Nkx 3.2, NK3 homeobox 2, NKX3 2, NKX3.2, NKX3-2, NKX3B

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Affinity purification

Store at -20 degree C. Avoid freeze / thaw cycles.

Small volumes of anti-NKX3-2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This gene encodes a member of the NK family of homeobox-containing proteins. The encoded protein may play a role in skeletal development.

Polyclonal

Western Blot (WB)

WB: 1:200 - 1:1000

Western blot analysis of extracts of 293T cells, using NKX3-2 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Calculated MW: 35kDa
Molecular Weight: 333

NK3 homeobox 2

homeobox protein Nkx-3.2

Homeobox protein Nkx-3.2

BAPX1; NKX3B  [Similar Products]

NKX32_HUMAN

This gene encodes a member of the NK family of homeobox-containing proteins. The encoded protein may play a role in skeletal development. [provided by RefSeq, Jul 2008]

NKX3-2: Transcriptional repressor that acts as a negative regulator of chondrocyte maturation. PLays a role in distal stomach development; required for proper antral-pyloric morphogenesis and development of antral-type epithelium. In concert with GSC, defines the structural components of the middle ear; required for tympanic ring and gonium development and in the regulation of the width of the malleus. Defects in NKX3-2 are the cause of spondylo- megaepiphyseal-metaphyseal dysplasia (SMMD). It is a skeletal dysplasia characterized by disproportionate short stature with a short and stiff neck and trunk, relatively long limbs that may show flexion contractures of the distal joints, delayed and impaired ossification of the vertebral bodies, the presence of large epiphysea ossification centers and wide growth plates in the long tubular bones and numerous pseudoepiphyses of the short tubular bones in hands and feet. Belongs to the NK-3 homeobox family.

Protein type: Transcription factor; DNA-binding; Cell development/differentiation

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: nucleus

Biological Process: determination of left/right symmetry; embryonic skeletal development; middle ear morphogenesis; negative regulation of apoptosis; negative regulation of chondrocyte differentiation; negative regulation of transcription from RNA polymerase II promoter; organ formation; pancreas development; skeletal development; skeletal morphogenesis; spleen development; transcription from RNA polymerase II promoter

Disease: Spondylo-megaepiphyseal-metaphyseal Dysplasia

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