Product Name
CALCA, siRNA
Full Product Name
CALCA siRNA (Rat)
Product Synonym Names
CALC; Calcitonin
Product Gene Name
CALCA sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P01257
Specificity
CALCA siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat CALCA gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CALCA sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CALCA sirna
siRNA to inhibit CALCA expression using RNA interference
Applications Tested/Suitable for CALCA sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CALCA. It may not necessarily be applicable to this product.
NCBI Accession #
NP_059034.1
[Other Products]
NCBI GenBank Nucleotide #
NM_017338.2
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UniProt Primary Accession #
P01257
[Other Products]
UniProt Related Accession #
P01256; P01257[Other Products]
Molecular Weight
13,948 Da
NCBI Official Full Name
calcitonin gene-related peptide 1 isoform Calca preproprotein
NCBI Official Synonym Full Names
calcitonin-related polypeptide alpha
NCBI Official Symbol
Calca [Similar Products]
NCBI Official Synonym Symbols
CAL6; CGRP; Cal1; Calc; RATCAL6; calcitonin
[Similar Products]
NCBI Protein Information
calcitonin gene-related peptide 1
UniProt Protein Name
Calcitonin
Protein Family
Calcitonin
UniProt Gene Name
Calca [Similar Products]
UniProt Synonym Gene Names
Calc [Similar Products]
UniProt Entry Name
CALC_RAT
NCBI Summary for CALCA
an inhibitor of lactotroph function and a potent vasodilator [RGD, Feb 2006]
UniProt Comments for CALCA
CALCA: Calcitonin causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Belongs to the calcitonin family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Cellular Component: extracellular space; neuron projection; cell soma; cell; axon; cytoplasm; extracellular region; terminal button; intracellular; nucleus
Molecular Function: neuropeptide hormone activity; identical protein binding; calcitonin receptor binding; protein complex binding; receptor binding
Biological Process: negative regulation of osteoclast differentiation; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of cell adhesion; positive regulation of interleukin-1 alpha production; vasodilation of artery during baroreceptor response to increased systemic arterial blood pressure; response to pain; positive regulation of vasodilation; vasodilation; G-protein signaling, adenylate cyclase activating pathway; protein amino acid phosphorylation; regulation of heart contraction; positive regulation of interleukin-8 production; monocyte chemotaxis; leukocyte adhesion; elevation of cytosolic calcium ion concentration; vasculature development; neuropeptide signaling pathway; regulation of blood pressure; receptor internalization; negative regulation of smooth muscle contraction; positive regulation of cAMP biosynthetic process; negative regulation of ossification; feeding behavior; positive regulation of adenylate cyclase activity; negative regulation of blood pressure; positive regulation of macrophage differentiation; detection of temperature stimulus involved in sensory perception of pain; inflammatory response; negative regulation of bone resorption; negative regulation of neurological process; neuromuscular junction development; aging; cytosolic calcium ion homeostasis; regulation of heart rate; adenylate cyclase activation; positive regulation of ossification; cellular calcium ion homeostasis; regulation of systemic arterial blood pressure by neurological process; G-protein coupled receptor internalization; response to heat; endothelial cell proliferation; activation of protein kinase activity; endothelial cell migration; negative regulation of transcription, DNA-dependent; embryo implantation
Research Articles on CALCA
1. Studied the effect of type 2 diabetes induced by high-fat diet and streptozotocin, and the effect of endurance training on basal circulating levels of calcitonin gene-related peptide (CGRP) and lactate.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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