Full Product Name
MYH8 siRNA (Human)
Product Synonym Names
Myosin-8; Myosin heavy chain 8; Myosin heavy chain. skeletal muscle. perinatal; MyHC-perinatal
Product Gene Name
MYH8 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P13535
Specificity
MYH8 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MYH8 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of MYH8 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
MYH8 sirna
siRNA to inhibit MYH8 expression using RNA interference
Applications Tested/Suitable for MYH8 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for MYH8. It may not necessarily be applicable to this product.
NCBI Accession #
NP_002463.2
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NCBI GenBank Nucleotide #
NM_002472.2
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UniProt Primary Accession #
P13535
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UniProt Secondary Accession #
Q14910[Other Products]
UniProt Related Accession #
P13535[Other Products]
Molecular Weight
222,763 Da
NCBI Official Full Name
myosin-8
NCBI Official Synonym Full Names
myosin, heavy chain 8, skeletal muscle, perinatal
NCBI Official Symbol
MYH8 [Similar Products]
NCBI Official Synonym Symbols
DA7; MyHC-pn; gtMHC-F; MyHC-peri
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NCBI Protein Information
myosin-8
UniProt Protein Name
Myosin-8
UniProt Synonym Protein Names
Myosin heavy chain 8; Myosin heavy chain, skeletal muscle, perinatal; MyHC-perinatal
UniProt Gene Name
MYH8 [Similar Products]
UniProt Synonym Gene Names
MyHC-perinatal [Similar Products]
UniProt Entry Name
MYH8_HUMAN
NCBI Summary for MYH8
Myosins are actin-based motor proteins that function in the generation of mechanical force in eukaryotic cells. Muscle myosins are heterohexamers composed of 2 myosin heavy chains and 2 pairs of nonidentical myosin light chains. This gene encodes a member of the class II or conventional myosin heavy chains, and functions in skeletal muscle contraction. This gene is predominantly expressed in fetal skeletal muscle. This gene is found in a cluster of myosin heavy chain genes on chromosome 17. A mutation in this gene results in trismus-pseudocamptodactyly syndrome. [provided by RefSeq, Sep 2009]
UniProt Comments for MYH8
MYH8: Muscle contraction. Defects in MYH8 are a cause of Carney complex variant (CACOV). Carney complex is a multiple neoplasia syndrome characterized by spotty skin pigmentation, cardiac and other myxomas, endocrine tumors, and psammomatous melanotic schwannomas. Familial cardiac myxomas are associated with spotty pigmentation of the skin and other phenotypes, including primary pigmented nodular adrenocortical dysplasia, extracardiac (frequently cutaneous) myxomas, schwannomas, and pituitary, thyroid, testicular, bone, ovarian, and breast tumors. Cardiac myxomas do not develop in all patients with the Carney complex, but affected patients have at least two features of the complex or one feature and a clinically significant family history. Defects in MYH8 are a cause of distal arthrogryposis type (DA7). A hereditary distal arthrogryposis characterized by an inability to open the mouth fully (trismus) and pseudocamptodactyly in which wrist dorsiflexion, but not volarflexion, produces involuntary flexion contracture of distal and proximal interphalangeal joints. Such hand and jaw contractures are caused by shortened flexor muscle-tendon units. Similar lower-limb contractures also produce foot deformity. The trismus-pseudocamptodactyly syndrome is a morbid autosomal dominant trait with variable expressivity but high penetrance. In these patients, trismus complicates dental care, feeding during infancy, and intubation for anesthesia, and the pseudocamptodactyly impairs manual dexterity, with consequent occupational and social disability. Many patients require surgical correction of contractures.
Protein type: Motor; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 17p13.1
Cellular Component: sarcomere; cytoplasm; muscle myosin complex; cytosol
Molecular Function: calmodulin binding; actin filament binding; microfilament motor activity; structural constituent of muscle; ATPase activity; myosin light chain binding; ATP binding
Biological Process: skeletal muscle contraction; muscle contraction; metabolic process; muscle filament sliding
Disease: Carney Complex Variant; Arthrogryposis, Distal, Type 7
Research Articles on MYH8
1. A mutation due to a possible germline mosaicism in MYH8">a family in which two out three sibs affected with trismus pseudocamptodactyly, born from healthy nonconsanguineous parents, were heterozygous for the c.2021G > A mutation due to a possible germline mosaicism in MYH8
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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