CD41, Monoclonal Antibody

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG1

PM6/248

Mouse

Preservative Free
Purified IgG - liquid

IgG concentration 1.0 mg/ml (lot specific)

Store at -20 degree C only. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Small volumes of anti-CD41 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Mouse anti Human CD41 antibody, clone PM6/248 recognizes the human CD41 cell surface antigen, a 140kD glycoprotein expressed by platelets and megakaryocytes. CD41 is also known as platelet glycoprotein IIb, and functions as a receptor for fibrinogen, fibronectin and vWF. It has not been established if clone PM6/248 recognizes free CD41 or CD41 only when complexed with CD61. However, antibody binding is reduced in the presence of EDTA suggesting that the epitope recognized is dependent upon an intact CD41/61 complex.

Flow cytometry (FC/FACS)

Flow Cytometry: Use 10ul of the suggested working dilution to label 100ul human whole blood.
Flow Cytometry: Minimum Dilution: 1/100; Maximum Dilution: 1/200

Staining of human peripheral blood platelets with Mouse anti Human CD41:RPE (MBS213833)

Staining of human peripheral blood platelets with Mouse anti Human CD41:Alexa Fluor 647

Staining of human peripheral blood platelets with Mouse anti Human CD41:FITC (MBS211023)

103,218 Da

integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41)

integrin alpha-IIb; GPalpha IIb; platelet fibrinogen receptor, alpha subunit; platelet membrane glycoprotein IIb; platelet-specific antigen BAK; protein phosphatase 1, regulatory subunit 93

Integrin alpha-IIb

GP2B; ITGAB; GPIIb  [Similar Products]

ITA2B_HUMAN

ITGA2B encodes integrin alpha chain 2b. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 2b undergoes post-translational cleavage to yield disulfide-linked light and heavy chains that join with beta 3 to form a fibronectin receptor expressed in platelets that plays a crucial role in coagulation. Mutations that interfere with this role result in thrombasthenia. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq, Jul 2008]

ITGA2B: Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha- IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface. Belongs to the integrin alpha chain family. Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-IIb associates with beta-3. Directly interacts with RNF181. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: platelet alpha granule membrane; cell surface; focal adhesion; integral to plasma membrane; plasma membrane; integrin complex; external side of plasma membrane

Molecular Function: identical protein binding; protein binding; extracellular matrix binding; metal ion binding

Biological Process: integrin-mediated signaling pathway; axon guidance; platelet activation; extracellular matrix organization and biogenesis; platelet degranulation; cell-matrix adhesion; positive regulation of leukocyte migration; cell adhesion; blood coagulation

Disease: Glanzmann Thrombasthenia; Bleeding Disorder, Platelet-type, 16

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