WDR45, Polyclonal Antibody

Rabbit Anti-Human WDR45 Polyclonal; WIPI4 Antibody; WDR45 Antibody; WDRX1 Antibody; WD repeat domain phosphoinositide-interacting protein 4 Antibody; WIPI-4 Antibody; WDRXI4 Antibody; WD repeat-containing protein 45 Antibody; JM5 Antibody

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Detects ~40 kDa.

Peptide Affinity Purified

1 mg/ml (lot specific)

Store at -20 degree C.

Small volumes of anti-WDR45 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

WIPI-4 is a 360 amino acid protein containing three WD repeats. Existing as three alternatively spliced isoforms, WIPI-4 is ubiquitously expressed but found at highest levels in heart and skeletal muscle. WD repeat proteins are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Diseases associated with this gene include neurodegeneration with brain iron accululation 5 and 4.

Antibodies; Cancer; Autophagy; Cell Signaling; Apoptosis

Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

WB: 1:1000
ICC/IF: 1:100

Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of 40 kDa WDR45 protein using Rabbit Anti-WDR45 Polyclonal Antibody (SPC-651). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human 293Trap cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-WDR45 Polyclonal Antibody (SPC-651) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 40 kDa.

39,939 Da

WD repeat domain 45

WD repeat domain phosphoinositide-interacting protein 4

WD repeat domain phosphoinositide-interacting protein 4

WDRX1; WDRXI4; WIPI4; WIPI-4  [Similar Products]

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene has a pseudogene at chromosome 4q31.3. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity and full-length nature of some variants have not been determined. [provided by RefSeq, Jul 2008]

Plays an important role in the autophagy pathway, which is the major intracellular degradation system by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation.

1. http://www.genecards.org/cgi-bin/carddisp.pl?gene=WDR45 2. Strausberg R.L., et al. (2002) Proc Natl Acad Sci U S A. 99(26): 16899-16903. 3. Haack T.B., et al. (2012) Am J Hum Genet. 91(6): 1144-1149.

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