Product Name
ACTA2, siRNA
Full Product Name
ACTA2 siRNA (Human)
Product Synonym Names
ACTSA; ACTVS; Actin aortic smooth muscle; Alpha-actin-2; Cell growth-inhibiting gene 46 protein
Product Gene Name
ACTA2 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P62736
Specificity
ACTA2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ACTA2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ACTA2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ACTA2 sirna
siRNA to inhibit ACTA2 expression using RNA interference
Applications Tested/Suitable for ACTA2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ACTA2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001135417.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001141945.1
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UniProt Primary Accession #
P62736
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UniProt Secondary Accession #
P03996; P04108; Q6FI19; B2R8A4[Other Products]
UniProt Related Accession #
P62736[Other Products]
Molecular Weight
42,009 Da
NCBI Official Full Name
actin, aortic smooth muscle
NCBI Official Synonym Full Names
actin, alpha 2, smooth muscle, aorta
NCBI Official Symbol
ACTA2 [Similar Products]
NCBI Official Synonym Symbols
AAT6; ACTSA; MYMY5
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NCBI Protein Information
actin, aortic smooth muscle
UniProt Protein Name
Actin, aortic smooth muscle
UniProt Synonym Protein Names
Alpha-actin-2; Cell growth-inhibiting gene 46 protein
UniProt Gene Name
ACTA2 [Similar Products]
UniProt Synonym Gene Names
ACTSA; ACTVS [Similar Products]
UniProt Entry Name
ACTA_HUMAN
NCBI Summary for ACTA2
The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]
UniProt Comments for ACTA2
ACTA2: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Up-regulated in response to enterovirus 71 (EV71) infection. Belongs to the actin family.
Protein type: Cytoskeletal; Contractile; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 10q23.3
Cellular Component: extracellular space; protein complex; smooth muscle contractile fiber; cytoplasm; cytosol; actin cytoskeleton
Molecular Function: protein kinase binding; ATP binding
Biological Process: muscle contraction; regulation of blood pressure; response to virus; vascular smooth muscle contraction
Disease: Moyamoya Disease 5; Multisystemic Smooth Muscle Dysfunction Syndrome; Aortic Aneurysm, Familial Thoracic 6
Research Articles on ACTA2
1. We report a missense mutation in the smooth muscle alpha-actin (ACTA2; MIM*102620) gene in a 3 generational family from Northern Ireland in which iris flocculi were an ocular marker of the disease.
Precautions
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Disclaimer
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