Product Name
ABCA1, siRNA
Full Product Name
ABCA1 siRNA (Mouse)
Product Synonym Names
ABC1; ATP-binding cassette sub-family A member 1; ATP-binding cassette transporter 1; ABC-1; ATP-binding cassette 1
Product Gene Name
ABCA1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P41233
Specificity
ABCA1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ABCA1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ABCA1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ABCA1 sirna
siRNA to inhibit ABCA1 expression using RNA interference
Applications Tested/Suitable for ABCA1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ABCA1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_038482.3
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NCBI GenBank Nucleotide #
NM_013454.3
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UniProt Primary Accession #
P41233
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UniProt Secondary Accession #
B1AWZ8[Other Products]
UniProt Related Accession #
P41233[Other Products]
Molecular Weight
253,912 Da
NCBI Official Full Name
ATP-binding cassette sub-family A member 1
NCBI Official Synonym Full Names
ATP-binding cassette, sub-family A (ABC1), member 1
NCBI Official Symbol
Abca1 [Similar Products]
NCBI Official Synonym Symbols
Abc1; ABC-1
[Similar Products]
NCBI Protein Information
ATP-binding cassette sub-family A member 1
UniProt Protein Name
ATP-binding cassette sub-family A member 1
UniProt Synonym Protein Names
ATP-binding cassette transporter 1; ABC-1; ATP-binding cassette 1
Protein Family
ABC transporter A family
UniProt Gene Name
Abca1 [Similar Products]
UniProt Synonym Gene Names
Abc1; ABC-1; ATP-binding cassette 1 [Similar Products]
UniProt Entry Name
ABCA1_MOUSE
NCBI Summary for ABCA1
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. In humans, this protein functions as a cholesterol efflux pump in the cellular lipid removal pathway. Mutations in the human gene have been associated with Tangier's disease and familial high-density lipoprotein deficiency. [provided by RefSeq, Jul 2008]
UniProt Comments for ABCA1
ABCA1: an ATP-binding cassette (ABC) transporter that regulates cholesterol homeostasis and insulin secretion in beta-cells, and protects against cardiovascular disease. Inactivation of ABCA1 in beta-cells markedly impairs glucose tolerance and insulin secretion in mice. Rosiglitazone, an activator of the peroxisome proliferator-activated receptor-gamma, upregulates ABCA1 in beta-cells. ABCA1 expression in beta-cells is required for the beneficial effects of rosiglitazone on glucose tolerance. Mediates the transport of cholesterol and phospholipids from cells to HDL apolipoproteins, modulating HDL levels and atherogenesis. Induced by bacterial lipopolysaccharides (LPS) and repressed by ZNF202. Phosphorylation on Ser-2054 regulates phospholipid efflux. Unsaturated fatty acids destabilize ABCA1 by activating a PKC-delta pathway that phosphorylates ABCA1 serines. Defects in ABCA1 are a cause of high density lipoprotein deficiencies.
Protein type: Transporter, ABC family; Transporter; Membrane protein, multi-pass; Vesicle; Membrane protein, integral
Cellular Component: Golgi apparatus; cell surface; membrane; intracellular membrane-bound organelle; endocytic vesicle; perinuclear region of cytoplasm; integral to plasma membrane; integral to membrane; plasma membrane; phagocytic vesicle; lipid raft; external side of plasma membrane
Molecular Function: apolipoprotein A-I receptor activity; transporter activity; ATPase activity; nucleotide binding; apolipoprotein binding; ATPase binding; small GTPase binding; apolipoprotein A-I binding; protein binding; syntaxin binding; phospholipid transporter activity; ATPase activity, coupled to transmembrane movement of substances; cholesterol transporter activity; anion transmembrane transporter activity; ATP binding; receptor binding
Biological Process: endosome transport; platelet dense granule organization and biogenesis; phospholipid efflux; transport; positive regulation of cAMP biosynthetic process; protein amino acid lipidation; interleukin-1 beta secretion; transmembrane transport; peptide secretion; phospholipid homeostasis; cholesterol metabolic process; response to high density lipoprotein stimulus; regulation of Cdc42 protein signal transduction; cholesterol transport; lipoprotein metabolic process; cholesterol efflux; intracellular cholesterol transport; phospholipid translocation; G-protein coupled receptor protein signaling pathway; cholesterol homeostasis; reverse cholesterol transport; lysosome organization and biogenesis; lipoprotein biosynthetic process; phospholipid transport; phagocytosis, engulfment
Research Articles on ABCA1
1. paeonol retarded the progress of atherosclerosis in ApoE(/) mice and modulated the expression of CD36 and ABCA1 in aortas similarly to that observed in macrophages
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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