Product Name
CACNA2D1, siRNA
Full Product Name
CACNA2D1 siRNA (Human)
Product Synonym Names
CACNL2A; CCHL2A; MHS3; Voltage-dependent calcium channel subunit alpha-2/delta-1; Voltage-gated calcium channel subunit alpha-2/delta-1
Product Gene Name
CACNA2D1 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P54289
Specificity
CACNA2D1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CACNA2D1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CACNA2D1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CACNA2D1 sirna
siRNA to inhibit CACNA2D1 expression using RNA interference
Applications Tested/Suitable for CACNA2D1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CACNA2D1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000713.2
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NCBI GenBank Nucleotide #
NM_000722.3
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UniProt Primary Accession #
P54289
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UniProt Secondary Accession #
Q17R45; Q9UD80; Q9UD81; Q9UD82[Other Products]
UniProt Related Accession #
P54289[Other Products]
Molecular Weight
122,443 Da
NCBI Official Full Name
voltage-dependent calcium channel subunit alpha-2/delta-1 preproprotein
NCBI Official Synonym Full Names
calcium channel, voltage-dependent, alpha 2/delta subunit 1
NCBI Official Symbol
CACNA2D1 [Similar Products]
NCBI Official Synonym Symbols
CACNA2; CCHL2A; CACNL2A; LINC01112; lncRNA-N3
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NCBI Protein Information
voltage-dependent calcium channel subunit alpha-2/delta-1
UniProt Protein Name
Voltage-dependent calcium channel subunit alpha-2/delta-1
UniProt Synonym Protein Names
Voltage-gated calcium channel subunit alpha-2/delta-1
Protein Family
Voltage-dependent calcium channel
UniProt Gene Name
CACNA2D1 [Similar Products]
UniProt Synonym Gene Names
CACNL2A; CCHL2A; MHS3 [Similar Products]
UniProt Entry Name
CA2D1_HUMAN
NCBI Summary for CACNA2D1
The preproprotein encoded by this gene is cleaved into multiple chains that comprise the alpha-2 and delta subunits of the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization. Mutations in this gene can cause cardiac deficiencies, including Brugada syndrome and short QT syndrome. Alternate splicing results in multiple transcript variants, some of which may lack the delta subunit portion. [provided by RefSeq, Nov 2014]
UniProt Comments for CACNA2D1
CACNA2D1: The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Plays an important role in excitation-contraction coupling. Belongs to the calcium channel subunit alpha-2/delta family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Channel, calcium
Chromosomal Location of Human Ortholog: 7q21-q22
Cellular Component: sarcoplasmic reticulum; T-tubule; voltage-gated calcium channel complex
Molecular Function: voltage-gated calcium channel activity; metal ion binding
Biological Process: calcium ion transport; regulation of calcium ion transport
Research Articles on CACNA2D1
1. High prevalence of CACNA2D1, SCN5A, and CACNB2 genetic variants in the Danish population previously associated with Brugada syndrome has been found in new exome data.
Precautions
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Disclaimer
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