ACTH (adrenocorticotropic hormone), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of ACTH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7606874 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ACTH (adrenocorticotropic hormone) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ACTH. The ELISA analytical biochemical technique of the MBS7606874 kit is based on ACTH antibody-ACTH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ACTH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ACTH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with ACTH. During the reaction,ACTH in the sample or standard competes with a fixed amount ofACTH on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to ACTH. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ACTH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

26,707 Da

pro-opiomelanocortin-alpha

pro-opiomelanocortin

Pro-opiomelanocortin

Pomc1; POMC; ACTH; CLIP  [Similar Products]

This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing. Processing yields several biologically active peptides, which are involved in diverse cellular functions, such as energy homeostasis, steroidogenesis, and increased melanin production in melanocytes. In mouse deficiency of this gene is associated with obesity, defects in adrenal development, and altered pigmentation. A pseudogene of this gene is located on chromosome 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

POMC: ACTH stimulates the adrenal glands to release cortisol. Defects in POMC may be associated with susceptibility to obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Defects in POMC are the cause of pro-opiomelanocortinin deficiency (POMCD). Affected individuals present early-onset obesity, adrenal insufficiency and red hair. Belongs to the POMC family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12 A1.1|12 1.99 cM

Cellular Component: cytoplasm; extracellular region; extracellular space; secretory granule

Molecular Function: G-protein-coupled receptor binding; hormone activity; receptor binding; type 3 melanocortin receptor binding; type 4 melanocortin receptor binding

Biological Process: cell-cell signaling; cellular pigmentation; generation of precursor metabolites and energy; glucose homeostasis; negative regulation of tumor necrosis factor production; positive regulation of transcription from RNA polymerase II promoter; regulation of appetite; regulation of blood pressure; signal transduction

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