Full Product Name
FLT4 siRNA (Rat)
Product Synonym Names
FLT-4; VEGFR3; Vascular endothelial growth factor receptor 3; VEGFR-3; Fms-like tyrosine kinase 4; FLT-4; Tyrosine-protein kinase receptor FLT4
Product Gene Name
FLT4 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q91ZT1
Specificity
FLT4 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat FLT4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of FLT4 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
FLT4 sirna
siRNA to inhibit FLT4 expression using RNA interference
Applications Tested/Suitable for FLT4 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for FLT4. It may not necessarily be applicable to this product.
NCBI Accession #
NP_446104.1
[Other Products]
NCBI GenBank Nucleotide #
NM_053652.1
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UniProt Primary Accession #
Q91ZT1
[Other Products]
UniProt Secondary Accession #
O35755; Q91ZT0[Other Products]
UniProt Related Accession #
Q91ZT1[Other Products]
Molecular Weight
123,178 Da
NCBI Official Full Name
vascular endothelial growth factor receptor 3
NCBI Official Synonym Full Names
fms-related tyrosine kinase 4
NCBI Official Symbol
Flt4 [Similar Products]
NCBI Official Synonym Symbols
Vegfr3
[Similar Products]
NCBI Protein Information
vascular endothelial growth factor receptor 3
UniProt Protein Name
Vascular endothelial growth factor receptor 3
UniProt Synonym Protein Names
Fms-like tyrosine kinase 4; FLT-4; Tyrosine-protein kinase receptor FLT4
Protein Family
Vascular endothelial growth factor receptor
UniProt Gene Name
Flt4 [Similar Products]
UniProt Synonym Gene Names
Flt-4; Vegfr3; VEGFR-3; FLT-4 [Similar Products]
UniProt Entry Name
VGFR3_RAT
NCBI Summary for FLT4
VEGF receptor-related, cell surface associated kinase with growth promoting ability [RGD, Feb 2006]
UniProt Comments for FLT4
VEGFR3: Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD, and plays an essential role in ***** lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development. Promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting. Signaling by activated FLT4 leads to enhanced production of VEGFC, and to a lesser degree VEGFA, thereby creating a positive feedback loop that enhances FLT4 signaling. Modulates KDR signaling by forming heterodimers. The secreted isoform 3 may function as a decoy receptor for VEGFC and/or VEGFD and play an important role as a negative regulator of VEGFC-mediated lymphangiogenesis and angiogenesis. Binding of vascular growth factors to isoform 1 or isoform 2 leads to the activation of several signaling cascades; isoform 2 seems to be less efficient in signal transduction, because it has a truncated C-terminus and therefore lacks several phosphorylation sites. Mediates activation of the MAPK1/ERK2, MAPK3/ERK1 signaling pathway, of MAPK8 and the JUN signaling pathway, and of the AKT1 signaling pathway. Phosphorylates SHC1. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3- kinase. Promotes phosphorylation of MAPK8 at 'Thr-183' and 'Tyr- 185', and of AKT1 at 'Ser-473'. Interacts with VEGFC and VEGFD. Monomer in the absence of bound VEGFC or VEGFD. Homodimer in the presence of bound VEGFC or VEGFD. Can also form a heterodimer with KDR. Interacts with PTPN14; the interaction is enhanced by stimulation with VEGFC. Interacts with CRK, GRB2, PTK2/FAK1, SHC1, PIK3R1 and PTPN11/SHP- 2. Identified in a complex with SRC and ITGB1. Detected in endothelial cells. Widely expressed. Detected in fetal spleen, lung and brain. Detected in ***** liver, muscle, thymus, placenta, lung, testis, ovary, prostate, heart, and kidney. Present in an inactive conformation in the absence of bound ligand. Binding of VEGFC or VEGFD leads to dimerization and activation by autophosphorylation on tyrosine residues. Inhibited by MAZ51. Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (receptor); Membrane protein, integral; EC 2.7.10.1; TK group; VEGFR family
Cellular Component: integral to plasma membrane; cytoplasm; plasma membrane; nucleus; receptor complex
Molecular Function: vascular endothelial growth factor receptor activity; growth factor binding; transmembrane receptor protein tyrosine kinase activity; protein phosphatase binding; ATP binding
Biological Process: peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; positive regulation of JNK cascade; positive regulation of cell growth; positive regulation of MAPKKK cascade; vasculature development; positive regulation of cell proliferation; lymphangiogenesis; blood vessel morphogenesis; positive regulation of endothelial cell proliferation; positive regulation of protein amino acid phosphorylation; sprouting angiogenesis; lymph vessel development; vascular endothelial growth factor receptor signaling pathway; negative regulation of apoptosis
Research Articles on FLT4
1. Expression of VEGFR-3 is enhanced in various cell types of the infarcted heart.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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