FLT4 / VEGFR3, Monoclonal Antibody

Mouse Monoclonal [clone 818CT12.1.1] (IgG2a) to Human FLT4 / VEGFR3; Human FLT4 / VEGFR3; FLT41; Fms-related tyrosine kinase 4; Fms-like tyrosine kinase 4; LMPH1A; PCL; Soluble VEGFR3 variant 1; Vegf-r3; VEGFR3; Soluble VEGFR3 variant 2; Soluble VEGFR3 variant 3; FLT-4; Quek2; VEGF Receptor 3; VEGFR-3

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG2a

818CT12.1.1

Mouse

Human FLT4 / VEGFR3

Protein G purified

PBS, 0.09% sodium azide.

Short term 4 degree C
Long term aliquot and store at -20 degree C
Avoid freeze thaw cycles.

Small volumes of anti-FLT4 / VEGFR3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

FLT4 Antibody, FLT41 Antibody, Fms-related tyrosine kinase 4 Antibody, Fms-like tyrosine kinase 4 Antibody, LMPH1A Antibody, PCL Antibody, Soluble VEGFR3 variant 1 Antibody, Vegf-r3 Antibody, VEGFR3 Antibody, Soluble VEGFR3 variant 2 Antibody, Soluble VEGFR3 variant 3 Antibody, FLT-4 Antibody, Quek2 Antibody, VEGF Receptor 3 Antibody, VEGFR-3 Antibody Description: Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD, and plays an essential role in ***** lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development. Promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting.

Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

IHC-P (1:50)
WB (1:2000)

Anti-FLT4 / VEGFR3 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.

VEGFR3 Antibody western blot of 293 and A549 cell line lysates (35 ug/lane). The VEGFR3 antibody detected the VEGFR3 protein (arrow).

Flow cytometric of HUVEC cells using VEGFR3 (green) compared to an isotype control of mouse IgG2a(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.

93,174 Da

fms related tyrosine kinase 4

vascular endothelial growth factor receptor 3

Vascular endothelial growth factor receptor 3

VEGFR3; VEGFR-3; FLT-4  [Similar Products]

VGFR3_HUMAN

This gene encodes a tyrosine kinase receptor for vascular endothelial growth factors C and D. The protein is thought to be involved in lymphangiogenesis and maintenance of the lymphatic endothelium. Mutations in this gene cause hereditary lymphedema type IA. [provided by RefSeq, Jul 2008]

VEGFR3: Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD, and plays an essential role in ***** lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development. Promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting. Signaling by activated FLT4 leads to enhanced production of VEGFC, and to a lesser degree VEGFA, thereby creating a positive feedback loop that enhances FLT4 signaling. Modulates KDR signaling by forming heterodimers. The secreted isoform 3 may function as a decoy receptor for VEGFC and/or VEGFD and play an important role as a negative regulator of VEGFC-mediated lymphangiogenesis and angiogenesis. Binding of vascular growth factors to isoform 1 or isoform 2 leads to the activation of several signaling cascades; isoform 2 seems to be less efficient in signal transduction, because it has a truncated C-terminus and therefore lacks several phosphorylation sites. Mediates activation of the MAPK1/ERK2, MAPK3/ERK1 signaling pathway, of MAPK8 and the JUN signaling pathway, and of the AKT1 signaling pathway. Phosphorylates SHC1. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3- kinase. Promotes phosphorylation of MAPK8 at 'Thr-183' and 'Tyr- 185', and of AKT1 at 'Ser-473'. Interacts with VEGFC and VEGFD. Monomer in the absence of bound VEGFC or VEGFD. Homodimer in the presence of bound VEGFC or VEGFD. Can also form a heterodimer with KDR. Interacts with PTPN14; the interaction is enhanced by stimulation with VEGFC. Interacts with CRK, GRB2, PTK2/FAK1, SHC1, PIK3R1 and PTPN11/SHP- 2. Identified in a complex with SRC and ITGB1. Detected in endothelial cells. Widely expressed. Detected in fetal spleen, lung and brain. Detected in ***** liver, muscle, thymus, placenta, lung, testis, ovary, prostate, heart, and kidney. Present in an inactive conformation in the absence of bound ligand. Binding of VEGFC or VEGFD leads to dimerization and activation by autophosphorylation on tyrosine residues. Inhibited by MAZ51. Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; EC 2.7.10.1; Protein kinase, TK; Protein kinase, tyrosine (receptor); Membrane protein, integral; TK group; VEGFR family

Chromosomal Location of Human Ortholog: 5q35.3

Cellular Component: cytoplasm; extracellular region; integral to plasma membrane; nucleus; plasma membrane; receptor complex

Molecular Function: ATP binding; growth factor binding; protein binding; protein phosphatase binding; transmembrane receptor protein tyrosine kinase activity; vascular endothelial growth factor receptor activity

Biological Process: blood vessel morphogenesis; lymph vessel development; lymphangiogenesis; negative regulation of apoptosis; peptidyl-tyrosine phosphorylation; positive regulation of cell proliferation; positive regulation of endothelial cell proliferation; positive regulation of JNK cascade; positive regulation of MAPKKK cascade; positive regulation of protein amino acid phosphorylation; protein amino acid autophosphorylation; sprouting angiogenesis; transmembrane receptor protein tyrosine kinase signaling pathway; vascular endothelial growth factor receptor signaling pathway; vasculature development

Disease: Hemangioma, Capillary Infantile; Lymphedema, Hereditary, Ia

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