Product Name
PCDH15, siRNA
Full Product Name
PCDH15 siRNA (Human)
Product Synonym Names
USH1F; Protocadherin-15
Product Gene Name
PCDH15 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q96QU1
Specificity
PCDH15 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PCDH15 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of PCDH15 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
PCDH15 sirna
siRNA to inhibit PCDH15 expression using RNA interference
Applications Tested/Suitable for PCDH15 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for PCDH15. It may not necessarily be applicable to this product.
NCBI Accession #
NP_149045.3
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NCBI GenBank Nucleotide #
NM_033056.3
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UniProt Primary Accession #
Q96QU1
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UniProt Secondary Accession #
Q5VY38; Q5VY39; Q6TRH8; Q8NDB9; Q96QT8; A6NL19; C6ZEF5; C6ZEF6; C6ZEF7[Other Products]
UniProt Related Accession #
Q96QU1[Other Products]
Molecular Weight
185,964 Da
NCBI Official Full Name
protocadherin-15 isoform CD1-4
NCBI Official Synonym Full Names
protocadherin-related 15
NCBI Official Symbol
PCDH15 [Similar Products]
NCBI Official Synonym Symbols
USH1F; CDHR15; DFNB23
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NCBI Protein Information
protocadherin-15
UniProt Protein Name
Protocadherin-15
Protein Family
Protocadherin
UniProt Gene Name
PCDH15 [Similar Products]
UniProt Synonym Gene Names
USH1F [Similar Products]
UniProt Entry Name
PCD15_HUMAN
NCBI Summary for PCDH15
This gene is a member of the cadherin superfamily. Family members encode integral membrane proteins that mediate calcium-dependent cell-cell adhesion. It plays an essential role in maintenance of normal retinal and cochlear function. Mutations in this gene result in hearing loss and Usher Syndrome Type IF (USH1F). Extensive alternative splicing resulting in multiple isoforms has been observed in the mouse ortholog. Similar alternatively spliced transcripts are inferred to occur in human, and additional variants are likely to occur. [provided by RefSeq, Dec 2008]
UniProt Comments for PCDH15
PCDH15: Calcium-dependent cell-adhesion protein. Essential for maintenance of normal retinal and cochlear function. Defects in PCDH15 are the cause of Usher syndrome type 1F (USH1F). USH is a genetically heterogeneous condition characterized by the association of retinitis pigmentosa and sensorineural deafness. Age at onset and differences in auditory and vestibular function distinguish Usher syndrome type 1 (USH1), Usher syndrome type 2 (USH2) and Usher syndrome type 3 (USH3). USH1 is characterized by profound congenital sensorineural deafness, absent vestibular function and prepubertal onset of progressive retinitis pigmentosa leading to blindness. Defects in PCDH15 are a cause of Usher syndrome type 1D/F (USH1DF). USH1DF patients are heterozygous for mutations in CDH23 and PCDH15, indicating a digenic inheritance pattern. Defects in PCDH15 are the cause of deafness autosomal recessive type 23 (DFNB23). DFNB23 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Cell adhesion; Cell development/differentiation
Chromosomal Location of Human Ortholog: 10q21.1
Cellular Component: stereocilium; extracellular space; photoreceptor outer segment; integral to membrane; plasma membrane; synapse
Molecular Function: calcium ion binding
Biological Process: sensory perception of sound; sensory perception of light stimulus; photoreceptor cell maintenance; homophilic cell adhesion; equilibrioception
Disease: Usher Syndrome, Type If; Usher Syndrome, Type Id; Deafness, Autosomal Recessive 23
Research Articles on PCDH15
1. PCDH15 has several alternate cytoplasmic domain exons and differentially spliced isoforms may function redundantly
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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