14-3-3 beta, Polyclonal Antibody

14-3-3 b Antibody; 14-3-3 protein beta/alpha; 1433B; 143B; KCIP-1; Protein 1054

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

The antibody detects endogenous levels of total 14-3-3 b protein.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

1.0 mg/ml (lot specific)

Store at -20 degree C

Small volumes of anti-14-3-3 beta antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2.Leffers H., J. Mol. Biol. 231:982-998(1993).Deloukas P., Nature 414:865-871(2001).Gevaert K., Nat. Biotechnol. 21:566-569(2003).Chi A., J. Proteome Res. 5:3135-3144(2006).

Total protein Ab

Western Blot (WB)

Western blotting: 1:500~1:3000

Western blot analysis of extracts from HepG2 cells and Jurkat cells, using 14-3-3 b antibody.

27,850 Da

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta

14-3-3 protein beta/alpha

14-3-3 protein beta/alpha

KCIP-1  [Similar Products]

1433B_HUMAN

This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]

14-3-3 beta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 20q13.1

Cellular Component: cytoplasmic vesicle membrane; focal adhesion; membrane; transcriptional repressor complex; perinuclear region of cytoplasm; cytoplasm; melanosome; nucleus; cytosol

Molecular Function: protein C-terminus binding; protein domain specific binding; protein binding; enzyme binding; histone deacetylase binding; protein complex binding; phosphoprotein binding; transcription corepressor activity; phosphoserine binding

Biological Process: negative regulation of protein amino acid dephosphorylation; positive regulation of catalytic activity; epidermal growth factor receptor signaling pathway; axon guidance; fibroblast growth factor receptor signaling pathway; activation of MAPKK activity; nerve growth factor receptor signaling pathway; protein heterooligomerization; apoptosis; MAPKKK cascade; small GTPase mediated signal transduction; Ras protein signal transduction; insulin receptor signaling pathway; cytoplasmic sequestering of protein; innate immune response; gene expression; negative regulation of transcription, DNA-dependent; vascular endothelial growth factor receptor signaling pathway; protein targeting

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