amine oxidase, copper containing 3 (vascular adhesion protein 1), ELISA Kit

Mouse Membrane primary amine oxidase (AOC3) ELISA kit; HPAO; SSAO; VAP-1; VAP1; amine oxidase; copper containing 3; copper amine oxidase; semicarbazide-sensitive amine oxidase; vascular adhesion protein 1; amine oxidase; copper containing 3 (vascular adhesion protein 1)

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Mouse AOC3. No significant cross-reactivity or interference between Mouse AOC3 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of AOC3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS928369 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the amine oxidase, copper containing 3 (vascular adhesion protein 1) (AOC3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AOC3. The ELISA analytical biochemical technique of the MBS928369 kit is based on AOC3 antibody-AOC3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AOC3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AOC3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AOC3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AOC3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AOC3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AOC3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

84,534 Da

amine oxidase, copper containing 3

membrane primary amine oxidase; VAP-1; copper amine oxidase; vascular adhesion protein 1; semicarbazide-sensitive amine oxidase

Membrane primary amine oxidase

Vap1; SSAO; VAP-1  [Similar Products]

AOC3_MOUSE

AOC3: Cell adhesion protein that participates in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin- independent fashion. Has a monoamine oxidase activity. May play a role in adipogenesis. Belongs to the copper/topaquinone oxidase family.

Protein type: Amino Acid Metabolism - phenylalanine; Other Amino Acids Metabolism - beta-alanine; Amino Acid Metabolism - tyrosine; Cell adhesion; EC 1.4.3.21; Oxidoreductase; Membrane protein, integral; Amino Acid Metabolism - glycine, serine and threonine

Cellular Component: extracellular space; microvillus; cell surface; membrane; intracellular membrane-bound organelle; cytoplasm; plasma membrane; integral to membrane

Molecular Function: amine oxidase activity; protein homodimerization activity; copper ion binding; protein heterodimerization activity; metal ion binding; oxidoreductase activity; cation channel activity; calcium ion binding; quinone binding

Biological Process: response to antibiotic; eating behavior; positive regulation of acute inflammatory response; positive regulation of leukocyte migration; regulation of blood pressure; amine metabolic process; cell adhesion; cation transport; leukocyte migration during inflammatory response

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.