Alpha-Crosslaps, ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C

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Small volumes of alphaCTx elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS084319 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Alpha-Crosslaps (alphaCTx) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing alphaCTx. The ELISA analytical biochemical technique of the MBS084319 kit is based on alphaCTx antibody-alphaCTx antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect alphaCTx antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, alphaCTx. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

278,303 Da

alpha Spectrin

CG1977 gene product from transcript CG1977-RA; CG1977-PA; CG1977-PB; CG1977-PC; CG1977-PD; aSpc 3A9; alpha spectrin; alpha-SPECTRIN; alpha-Spec-PA; alpha-Spec-PB; alpha-Spec-PC; alpha-Spec-PD; alpha-spectrin II; alphaSpectrin; anon-fast-evolving-2E2; klotzchen

Spectrin alpha chain

SPEC-A  [Similar Products]

SPTCA_DROME

Function: Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane. Essential for larval survival and development. Stabilizes cell to cell interactions that are critical for the maintenance of cell shape and subcellular organization within embryonic tissues. Lva and spectrin may form a Golgi-based scaffold that mediates interaction of Golgi bodies with microtubules and facilitates Golgi-derived membrane secretion required for the formation of furrows during cellularization. Ref.5 Ref.7 Ref.8

Subunit structure: Native spectrin molecule is a tetramer composed of two antiparallel heterodimers joined head to head so that each end of the native molecule includes the C-terminus of the alpha subunit and the N-terminus of the beta subunit. Interacts with calmodulin in a calcium-dependent manner, interacts with F-actin and also interacts with Lva. Interacts with Ten-m. Ref.8 Ref.13

Subcellular location: Cytoplasm › cytoskeleton. Golgi apparatus. Note: Near the inner surface of the plasma membrane of nearly all cells. Lva-alpha-spectrin complexes are found at the Golgi. Ref.8

Tissue specificity: A substantial pool of maternal protein in the egg undergoes dynamic changes in distribution early in embryogenesis. In gastrulated embryo, the highest level of protein is found in the respiratory tract cells and the lowest in parts of the forming gut. Ref.9

Developmental stage: Expressed both maternally and zygotically.

Miscellaneous: Its transcript shares the first untranslated exon with the dlt transcript, suggesting a common regulation.

Sequence similarities: Belongs to the spectrin family.Contains 2 EF-hand domains.Contains 1 SH3 domain.Contains 22 spectrin repeats.

Sequence caution: The sequence AAL39886.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence ABA81823.1 differs from that shown. Reason: Frameshift at position 1549.

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