COMMD1, Polyclonal Antibody

MURR1; C2orf5

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Affinity Purification

1mg/ml (lot specific)

Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Small volumes of anti-COMMD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

COMMD1 is a regulator of copper homeostasis, sodium uptake, and NF-kappa-B (see MIM 164011) signaling (de Bie et al., 2005

Polyclonal

Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

WB: 1:1000-1:2000
IP: 1:20-1:50
IHC: 1:50-1:200

Western blot analysis of extracts of various cell lines, using COMMD1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

190

copper metabolism (Murr1) domain containing 1

COMM domain-containing protein 1; protein Murr1; copper metabolism gene MURR1

COMM domain-containing protein 1

C2orf5; MURR1  [Similar Products]

COMD1_HUMAN

COMMD1 is a regulator of copper homeostasis, sodium uptake, and NF-kappa-B (see MIM 164011) signaling (de Bie et al., 2005 [PubMed 16267171]).[supplied by OMIM, Sep 2009]

COMMD1: Promotes ubiquitination of NF-kappa-B subunit RELA and its subsequent proteasomal degradation. Down-regulates NF-kappa-B activity. Down-regulates SOD1 activity by interfering with its homodimerization. Plays a role in copper ion homeostasis. Can bind one copper ion per monomer. May function to facilitate biliary copper excretion within hepatocytes.

Protein type: Inhibitor

Chromosomal Location of Human Ortholog: 2p15

Cellular Component: Cul2-RING ubiquitin ligase complex; nucleoplasm; recycling endosome; cytoplasm; early endosome; cytoplasmic membrane-bound vesicle; endosome membrane; nucleus

Molecular Function: identical protein binding; phosphatidylinositol-4,5-bisphosphate binding; protein binding; copper ion binding; protein homodimerization activity; phosphatidylinositol-3,4,5-triphosphate binding; phosphatidylinositol-3,4-bisphosphate binding

Biological Process: Golgi to plasma membrane transport; protein transport; transcription, DNA-dependent; positive regulation of protein ubiquitination; inhibition of NF-kappaB transcription factor; regulation of proteasomal ubiquitin-dependent protein catabolic process; plasma membrane to endosome transport; copper ion homeostasis

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