LRAT, Polyclonal Antibody

Lecithin retinol acyltransferase; EC 2.3.1.135; Phosphatidylcholine--retinol O-acyltransferase; LRAT

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

The antibody detects endogenous levels of total LRAT protein.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

1.0 mg/ml (lot specific)

Store at -20 degree C

Small volumes of anti-LRAT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Transfers the acyl group from the sn-1 position of phosphatidylcholine to all-trans retinol, producing all-trans retinyl esters. Retinyl esters are storage forms of vitamin A. LRAT plays a critical role in vision. It provides the all-trans retinyl ester substrates for the isomerohydrolase which processes the esters into 11-cis-retinol in the retinal pigment epithelium; due to a membrane-associated alcohol dehydrogenase, 11 cis-retinol is oxidized and converted into 11-cis-retinaldehyde which is the chromophore for rhodopsin and the cone photopigments.Ruiz A., J. Biol. Chem. 274:3834-3841(1999).Mondal M.S., Biochemistry 39:5215-5220(2000).Thompson D.A., Nat. Genet. 28:123-124(2001).

Total protein Ab

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500

Western blot analysis of extracts from COLO205 cells, using LRAT antibody.

Immunohistochemistry analysis of paraffin-embedded human prostate carcinoma tissue using LRAT antibody.

Immunofluorescence analysis of HUVEC cells, using LRAT antibody.

25,703 Da

lecithin retinol acyltransferase (phosphatidylcholine--retinol O-acyltransferase)

lecithin retinol acyltransferase

Lecithin retinol acyltransferase

LRAT_HUMAN

The protein encoded by this gene localizes to the endoplasmic reticulum, where it catalyzes the esterification of all-trans-retinol into all-trans-retinyl ester. This reaction is an important step in vitamin A metabolism in the visual system. Mutations in this gene have been associated with early-onset severe retinal dystrophy and Leber congenital amaurosis 14. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014]

LRAT: Transfers the acyl group from the sn-1 position of phosphatidylcholine to all-trans retinol, producing all-trans retinyl esters. Retinyl esters are storage forms of vitamin A. LRAT plays a critical role in vision. It provides the all-trans retinyl ester substrates for the isomerohydrolase which processes the esters into 11-cis-retinol in the retinal pigment epithelium; due to a membrane-associated alcohol dehydrogenase, 11 cis-retinol is oxidized and converted into 11-cis-retinaldehyde which is the chromophore for rhodopsin and the cone photopigments. Defects in LRAT are a cause of Leber congenital amaurosis type 14 (LCA14). It is a severe dystrophy of the retina, typically becoming evident in the first years of life. Visual function is usually poor and often accompanied by nystagmus, sluggish or near-absent pupillary responses, photophobia, high hyperopia and keratoconus. Belongs to the H-rev107 family.

Protein type: Cofactor and Vitamin Metabolism - retinol; Membrane protein, integral; Transferase; EC 2.3.1.135

Chromosomal Location of Human Ortholog: 4q32.1

Cellular Component: multivesicular body; endoplasmic reticulum membrane; rough endoplasmic reticulum; perinuclear region of cytoplasm; integral to membrane

Molecular Function: retinoic acid binding; transferase activity, transferring acyl groups; retinol binding; phosphatidylcholine-retinol O-acyltransferase activity

Biological Process: positive regulation of lipid transport; phototransduction, visible light; visual perception; retinol metabolic process; retinoic acid metabolic process; retinoid metabolic process; vitamin A metabolic process

Disease: Leber Congenital Amaurosis 14; Retinitis Pigmentosa

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