Full Product Name
RS1 siRNA (Human)
Product Synonym Names
XLRS1; Retinoschisin; X-linked juvenile retinoschisis protein
Product Gene Name
RS1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O15537
Specificity
RS1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RS1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of RS1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
RS1 sirna
siRNA to inhibit RS1 expression using RNA interference
Applications Tested/Suitable for RS1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for RS1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000321.1
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NCBI GenBank Nucleotide #
NM_000330.3
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UniProt Primary Accession #
O15537
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UniProt Secondary Accession #
Q0QD39[Other Products]
UniProt Related Accession #
O15537[Other Products]
Molecular Weight
25,592 Da
NCBI Official Full Name
retinoschisin
NCBI Official Synonym Full Names
retinoschisin 1
NCBI Official Symbol
RS1 [Similar Products]
NCBI Official Synonym Symbols
RS; XLRS1
[Similar Products]
NCBI Protein Information
retinoschisin
UniProt Protein Name
Retinoschisin
UniProt Synonym Protein Names
X-linked juvenile retinoschisis protein
Protein Family
Homeobox protein
UniProt Gene Name
RS1 [Similar Products]
UniProt Synonym Gene Names
XLRS1 [Similar Products]
UniProt Entry Name
XLRS1_HUMAN
NCBI Summary for RS1
This gene encodes an extracellular protein that plays a crucial role in the cellular organization of the retina. The encoded protein is assembled and secreted from photoreceptors and bipolar cells as a homo-oligomeric protein complex. Mutations in this gene are responsible for X-linked retinoschisis, a common, early-onset macular degeneration in males that results in a splitting of the inner layers of the retina and severe loss in vision. [provided by RefSeq, Oct 2008]
UniProt Comments for RS1
RS1: a highly conserved extracellular protein essential for proper retinal structure. Must interact with L-type voltage-gated calcium channels (L-VGCCs) correctly so that the photoreceptor cells in the eye can function properly. Genetic mutations in RS1 cause X-linked retinoschisis (XLRS) and early onset of macular degeneration. Interacts with the L-VGCCa1D subunit, regulating its activity. The expression and secretion of retinoschisin are modulated by circadian rhythms, peaking at night and diminishing during the day.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: Xp22.13
Cellular Component: extrinsic to plasma membrane; extracellular space
Molecular Function: phosphatidylinositol-4,5-bisphosphate binding; phosphatidylinositol-3,4,5-triphosphate binding; phosphatidylserine binding; phosphatidylinositol-5-phosphate binding; phosphatidylinositol-3,4-bisphosphate binding; phosphatidylinositol 3-phosphate binding
Biological Process: visual perception; multicellular organismal development; adaptation of rhodopsin mediated signaling; cell adhesion
Disease: Retinoschisis 1, X-linked, Juvenile
Research Articles on RS1
1. We identified a novel causative mutation of RS1 in a Chinese family with X-linked juvenile retinoschisis.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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