Caytaxin (ATCAY), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of ATCAY. No significant cross-reactivity or interference between ATCAY and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ATCAY and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of ATCAY elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7238763 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Caytaxin (ATCAY) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ATCAY. The ELISA analytical biochemical technique of the MBS7238763 kit is based on ATCAY antibody-ATCAY antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ATCAY antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ATCAY. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Intended Uses: This ATCAY ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ATCAY. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: ATCAY ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ATCAY antibody and an ATCAY-HRP conjugate. The assay sample and buffer are incubated together with ATCAY-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATCAY concentration since ATCAY from samples and ATCAY-HRP conjugate compete for the anti-ATCAY antibody . Since the number of sites is limited, as more sites are occupied by ATCAY from the sample, fewer sites are left to bind ATCAY-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATCAY concentration in each sample is interpolated from this standard curve.

Neurobiology

42,821 Da

ataxia, cerebellar, Cayman type

caytaxin; Cayman ataxia; BNIP-2-homolgy; BNIP-2-homology; ataxia cayman type protein

Caytaxin

KIAA1872; BNIP-H  [Similar Products]

ATCAY_HUMAN

This gene encodes a neuron-restricted protein that contains a CRAL-TRIO motif common to proteins that bind small lipophilic molecules. Mutations in this gene are associated with cerebellar ataxia, Cayman type. [provided by RefSeq, Jul 2008]

ATCAY: Functions in the development of neural tissues, particularly the postnatal maturation of the cerebellar cortex. May play a role in neurotransmission through regulation of glutaminase/GLS, an enzyme responsible for the production in neurons of the glutamate neurotransmitter. Alternatively, may regulate the localization of mitochondria within axons and dendrites. Defects in ATCAY are the cause of cerebellar ataxia, cayman type (ATCAY). ATCAY is found in a population isolate on Grand Cayman Island and causes a marked psychomotor retardation and prominent nonprogressive cerebellar dysfunction including nystagmus, intention tremor, dysarthria, and wide-based ataxic gait. Hypotonia is present from early childhood. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: neuron projection; mitochondrion; axon; cytoplasm; dendrite; mitochondrial membrane; synapse; cell junction

Molecular Function: protein binding; kinesin binding

Biological Process: regulation of protein localization; apoptosis; transport; neurite development; mitochondrion distribution

Disease: Cerebellar Ataxia, Cayman Type

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