Product Name
AMER1, siRNA
Full Product Name
AMER1 siRNA (Human)
Product Synonym Names
FAM123B; WTX; APC membrane recruitment protein 1; Amer1; Protein FAM123B; Wilms tumor gene on the X chromosome protein
Product Gene Name
AMER1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q5JTC6
Specificity
AMER1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human AMER1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of AMER1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
AMER1 sirna
siRNA to inhibit AMER1 expression using RNA interference
Applications Tested/Suitable for AMER1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for AMER1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_689637.3
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NCBI GenBank Nucleotide #
NM_152424.3
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UniProt Primary Accession #
Q5JTC6
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UniProt Secondary Accession #
Q8N885; A2IB86[Other Products]
UniProt Related Accession #
Q5JTC6[Other Products]
Molecular Weight
87,823 Da
NCBI Official Full Name
APC membrane recruitment protein 1
NCBI Official Synonym Full Names
APC membrane recruitment protein 1
NCBI Official Symbol
AMER1 [Similar Products]
NCBI Official Synonym Symbols
WTX; OSCS; FAM123B
[Similar Products]
NCBI Protein Information
APC membrane recruitment protein 1
UniProt Protein Name
APC membrane recruitment protein 1
UniProt Synonym Protein Names
Protein FAM123B; Wilms tumor gene on the X chromosome protein
Protein Family
APC membrane recruitment protein
UniProt Gene Name
AMER1 [Similar Products]
UniProt Synonym Gene Names
FAM123B; WTX; Amer1 [Similar Products]
UniProt Entry Name
AMER1_HUMAN
NCBI Summary for AMER1
The protein encoded by this gene upregulates trancriptional activation by the Wilms tumor protein and interacts with many other proteins, including CTNNB1, APC, AXIN1, and AXIN2. Defects in this gene are a cause of osteopathia striata with cranial sclerosis (OSCS). [provided by RefSeq, May 2010]
UniProt Comments for AMER1
WTX: a peripheral membrane protein on the cytoplasmic side that shuttles between nucleus and cytoplasm. Detected in nuclear paraspeckles that are found close to splicing speckles. Enhances trancription activation by WT1. Promotes CTNNB1 ubiquitination and degradation. Antagonizes Wnt and CTNNB1 signaling. Inactivated in approximately one-third of Wilms tumors. Detected in fetal and ***** kidney, brain and spleen. Involved in kidney development. Interacts with CTNNB1, AXIN1, KEAP1, APC and BTRC. Interacts with SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes containing BTRC and/or FBXW11. Identified in a complex containing CTNNB1, APC, AXIN1 and AXIN2. Two alternatively spliced human isoforms have been reported.
Chromosomal Location of Human Ortholog: Xq11.2
Cellular Component: plasma membrane; cytosol; nucleus
Molecular Function: protein binding; phosphatidylinositol-4,5-bisphosphate binding; beta-catenin binding
Biological Process: Wnt receptor signaling pathway
Disease: Osteopathia Striata With Cranial Sclerosis
Research Articles on AMER1
1. T, p.Glu349*) in the WTX gene.">A nonsense mutation (c.1045C>T, p.Glu349*) in the WTX gene.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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