Integrin beta3, Polyclonal Antibody

Integrin b3 Polyclonal Antibody; ITGB3; GP3A; Integrin beta-3; Platelet membrane glycoprotein IIIa; GPIIIa; CD antigen CD61

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Integrin b3 Polyclonal Antibody detects endogenous levels of Integrin b3 protein.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

1 mg/ml (lot specific)

Store at -20 degree C/1 year

Small volumes of anti-ITGB3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Total protein Ab

Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)

Western Blot: 1:500 - 1:2000
Immunohistochemistry: 1:100 - 1:300
ELISA: 1:10000
Not yet tested in other applications.

Western Blot analysis of NIH-3T3 cells using Integrin b3 Polyclonal Antibody

Western Blot analysis of HepG2-UV MOUSE-BRAIN cells using Integrin b3 Polyclonal Antibody

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ITB3_HUMAN

The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. [provided by RefSeq, Jul 2008]

ITGB3: integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIB/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIB/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIB/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha- IIB/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface.

Protein type: Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: filopodium membrane; platelet alpha granule membrane; focal adhesion; cell surface; microvillus membrane; integral to plasma membrane; melanosome; plasma membrane; integrin complex; nucleus; receptor complex

Molecular Function: identical protein binding; protein binding; protease binding; vascular endothelial growth factor receptor 2 binding; extracellular matrix binding; fibronectin binding; protein disulfide isomerase activity; platelet-derived growth factor receptor binding; receptor activity; cell adhesion molecule binding

Biological Process: negative regulation of lipid transport; axon guidance; extracellular matrix organization and biogenesis; entry of virus into host cell; wound healing; protein folding; cell-matrix adhesion; smooth muscle cell migration; mesodermal cell differentiation; negative chemotaxis; positive regulation of vascular endothelial growth factor receptor signaling pathway; platelet degranulation; cell-substrate junction assembly; cell adhesion; cell growth; integrin-mediated signaling pathway; platelet activation; cell migration; regulation of bone resorption; negative regulation of lipoprotein metabolic process; angiogenesis involved in wound healing; cell-substrate adhesion; heterotypic cell-cell adhesion; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of low-density lipoprotein receptor biosynthetic process; activation of protein kinase activity; positive regulation of endothelial cell proliferation; positive regulation of protein amino acid phosphorylation; blood coagulation; tube development; vascular endothelial growth factor receptor signaling pathway; leukocyte migration

Disease: Glanzmann Thrombasthenia; Myocardial Infarction, Susceptibility To; Bleeding Disorder, Platelet-type, 16

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