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cysteine-rich secretory protein 3, ELISA Kit

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产品名称: cysteine-rich secretory protein 3, ELISA Kit
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简单介绍

cysteine-rich secretory protein 3, ELISA Kit


cysteine-rich secretory protein 3, ELISA Kit  的详细介绍
Product Name

cysteine-rich secretory protein 3 (CRISP3), ELISA Kit

Popular Item
Full Product Name

Horse Cysteine-rich secretory protein 3, CRISP3 ELISA Kit

Product Synonym Names
Horse Cysteine-rich secretory protein 3 (CRISP3) ELISA kit; Aeg2; CRISP-3; CRS3; MGC126588; SGP28; dJ442L6.3; OTTHUMP00000016590; cysteine-rich secretory protein-3; specific granule protein (28 kDa) ; cysteine-rich secretory protein 3
Product Gene Name

CRISP3 elisa kit

[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Sample Manual Insert
Download Sample PDF Manual View Sample PDF Manual
Request for Current Manual Insert
Request Current Manual
3D Structure
ModBase 3D Structure for O19010
Species Reactivity
Horse
Specificity
This assay has high sensitivity and excellent specificity for detection of horse CRISP3. No significant cross-reactivity or interference between horse CRISP3 and analogues was observed.
Samples
Serum, plasma, tissue homogenates.
Assay Type
Competitive
Detection Range
0.156 ng/ml - 10 ng /ml.
Sensitivity
The minimum detectable dose of horse CRISP3 is typically less than 0.039 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CRISP3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for CRISP3 purchase
MBS905052 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cysteine-rich secretory protein 3 (CRISP3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CRISP3. The ELISA analytical biochemical technique of the MBS905052 kit is based on CRISP3 antibody-CRISP3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CRISP3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CRISP3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for
CRISP3 elisa kit
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with CRISP3. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for CRISP3. The competitive inhibition reaction is launched between with pre-coated CRISP3 and CRISP3 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of CRISP3 in the samples. The color development is stopped and the intensity of the color is measured.
NCBI/Uniprot data below describe general gene information for CRISP3. It may not necessarily be applicable to this product.
NCBI GI #
126352542
NCBI GeneID
100033965
NCBI Accession #
NP_001075343.1 [Other Products]
NCBI GenBank Nucleotide #
NM_001081874.1 [Other Products]
UniProt Primary Accession #
O19010 [Other Products]
UniProt Secondary Accession #
Q8HXA1[Other Products]
UniProt Related Accession #
O19010[Other Products]
Molecular Weight
27,308 Da
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NCBI Official Full Name
cysteine-rich secretory protein 3
NCBI Official Symbol
CRISP3  [Similar Products]
NCBI Official Synonym Symbols
AEG2; HSP-3
  [Similar Products]
NCBI Protein Information
cysteine-rich secretory protein 3; CRISP-3; horse seminal plasma protein 3; acidic epididymal glycoprotein 2
UniProt Protein Name
Cysteine-rich secretory protein 3
UniProt Synonym Protein Names
Acidic epididymal glycoprotein 2; AEG2
Protein Family
Cysteine-rich secretory protein
UniProt Gene Name
CRISP3  [Similar Products]
UniProt Synonym Gene Names
CRISP-3; AEG2  [Similar Products]
UniProt Entry Name
CRIS3_HORSE
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Research Articles on CRISP3
1. CRISP3 in seminal plasma suppresses polymorphonuclear neutrophil/sperm binding, suggesting that CRISP3 regulates sperm elimination from the female reproductive tract.
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Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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