Product Name
Cyt19/AS3MT, Polyclonal Antibody
Full Product Name
Goat anti-Cyt19/AS3MT Antibody
Product Synonym Names
AS3MT; CYT19; Cyt19; Cyt19 protein; likely ortholog of rat methyltransferase Cyt19; S-adenosylmethionine: arsenic (III) methyltransferase; arsenic (+3 oxidation state) methyltransferase; RP11-753C18. 6; 2310045H08Rik; methylarsonite methyltransferase; methy; AS3MT antibody; CYT19 antibody; Cyt19 antibody; Cyt19 protein antibody; likely ortholog of rat methyltransferase Cyt19 antibody; S-adenosylmethionine: arsenic (III) methyltransferase antibody; arsenic (+3 oxidation state) methyltransferase antibody; RP11-753C18. 6 antibody; 2310045H08Rik antibody; methylarsonite methyltransferase antibody; methyltransferase cyt19 antibody; Cyt19 / AS3MT
Product Gene Name
anti-AS3MT antibody
[Similar Products]
Product Synonym Gene Name
Cyt19[Similar Products]
Antibody/Peptide Pairs
Cyt19 / AS3MT peptide (MBS425230) is used for blocking the activity of Cyt19/AS3MT antibody (MBS420776)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
AALRDAEIQK DVQ
Species Reactivity
Expected from sequence similarity: Human
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence AALRDAEIQKDVQ-C, from the N Terminus of the protein sequence according to NP_065733.2.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-AS3MT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-AS3MT antibody
Peptide ELISA (EIA), Immunohistochemistry (IHC)
Application Notes for anti-AS3MT antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Immunohistochemistry: In paraffin embedded Human Kidney shows cytoplasm staining of epithelial cells with some emphasis on the luminal side of the DCT. Recommended concentration, 3-6ug/ml.
Immunohistochemistry (IHC) of anti-AS3MT antibody
(3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
NCBI/Uniprot data below describe general gene information for AS3MT. It may not necessarily be applicable to this product.
NCBI Accession #
NP_065733.2
[Other Products]
NCBI GenBank Nucleotide #
NM_020682.3
[Other Products]
UniProt Secondary Accession #
Q0VDK3; Q0VDK4; Q5PZ02; A6NP79[Other Products]
UniProt Related Accession #
Q9HBK9[Other Products]
Molecular Weight
31,098 Da
NCBI Official Full Name
arsenite methyltransferase
NCBI Official Synonym Full Names
arsenite methyltransferase
NCBI Official Symbol
AS3MT [Similar Products]
NCBI Official Synonym Symbols
CYT19
[Similar Products]
NCBI Protein Information
arsenite methyltransferase
UniProt Protein Name
Arsenite methyltransferase
UniProt Synonym Protein Names
Methylarsonite methyltransferase; S-adenosyl-L-methionine:arsenic(III) methyltransferase
Protein Family
Arsenite methyltransferase
UniProt Gene Name
AS3MT [Similar Products]
UniProt Synonym Gene Names
CYT19 [Similar Products]
UniProt Entry Name
AS3MT_HUMAN
NCBI Summary for AS3MT
AS3MT catalyzes the transfer of a methyl group from S-adenosyl-L-methionine (AdoMet) to trivalent arsenical and may play a role in arsenic metabolism (Lin et al., 2002 [PubMed 11790780]).[supplied by OMIM, Mar 2008]
UniProt Comments for AS3MT
AS3MT: Catalyzes the transfer of a methyl group from AdoMet to trivalent arsenicals producing methylated and dimethylated arsenicals. It methylates arsenite to form methylarsonate, Me- AsO(3)H(2), which is reduced by methylarsonate reductase to methylarsonite, Me-As(OH)2. Methylarsonite is also a substrate and it is converted into the much less toxic compound dimethylarsinate (cacodylate), Me(2)As(O)-OH. Belongs to the methyltransferase superfamily.
Protein type: Mitochondrial; EC 2.1.1.137; Methyltransferase
Chromosomal Location of Human Ortholog: 10q24.32
Cellular Component: cytoplasm; cytosol; mitochondrion
Molecular Function: arsenite methyltransferase activity; methylarsonite methyltransferase activity; S-adenosylmethionine-dependent methyltransferase activity
Biological Process: arsonoacetate metabolic process; methylation; toxin metabolic process
Research Articles on AS3MT
1. The present study found that rs3740400 in As3MT and rs156697 in GSTO2 were strongly associated with the arsenic species (%iAs, %MMA and MMA/iAs and DMA/MMA ratios) in urine of the occupationally exposed workers and they may influence methylation capacity.
Precautions
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