Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog, ELISA Ki

For Research Use Only. Not for use in diagnostic procedures.

This assay recognizes recombinant and natural mouse LPS-induced TNF-alpha factor homolog. No significant cross-reactivity or interference was observed.

For long term storage, please store the entire kit at -20 degree C.

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Small volumes of Litaf elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS288002 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog (Litaf) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Litaf. The ELISA analytical biochemical technique of the MBS288002 kit is based on Litaf antibody-Litaf antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Litaf antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Litaf. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LPS-induced TNF-alpha factor homolog. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for LPS-induced TNF-alpha factor homolog and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain LPS-induced TNF-alpha factor homolog, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of LPS-induced TNF-alpha factor homolog in the samples is then determined by comparing the O.D. of the samples to the standard curve.

16,946 Da

LPS-induced TN factor

lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog

Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog

N4wbp3; Tbx1; LPS-induced TNF-alpha factor homolog; mEET  [Similar Products]

LITAF_MOUSE

LITAF: Probable role in regulating transcription of specific genes. May regulate through NFKB1 the expression of the CCL2/MCP-1 chemokine. May play a role in tumor necrosis factor alpha (TNF- alpha) gene expression. Interacts with NEDD4. Interacts with WWOX. Isoform 2 may interact with STAT6. By bacterial lipopolysaccharides (LPS) or p53/TP53. In monocytes by the Bacillus Calmette-Guerin (BCG). Ubiquitously and abundantly expressed. Expressed predominantly in the placenta, peripheral blood leukocytes, lymph nodes and spleen. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; Transcription factor

Cellular Component: cytoplasm; Golgi apparatus; intracellular membrane-bound organelle; lysosomal membrane; lysosome; membrane; nucleoplasm; plasma membrane

Molecular Function: protein binding; signal transducer activity; WW domain binding

Biological Process: apoptosis; negative regulation of NF-kappaB import into nucleus; positive regulation of I-kappaB kinase/NF-kappaB cascade; regulation of cytokine production; regulation of transcription, DNA-dependent; response to lipopolysaccharide; signal transduction; transcription, DNA-dependent

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