INI-1/SNF5, Polyclonal Antibody

SMARCB1; BAF47; INI1; SNF5L1; BRG1-associated factor 47; Integrase interactor 1 protein; hSNF5; SNF5 homolog; Antibody to INI-1/SNF5; Anti-INI-1/SNF5 Antibody; INI-1/SNF5 Antibody

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Purified
Protein A Chromatography

25 ug in 50 ul/100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Store the antibody at 4 degree C, stable for 6 months.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.

Small volumes of anti-INI-1/SNF5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

SNF5/INI1 is the core subunit of the SWI/SNF chromatin remodeling complex that contributes to the regulation of chromatin structure. It is a tumor suppressor in humans and mice, with an essential role in early embryonic development. SNF5 interacts with UV damage recognition factor XPC and colocalizes at the damage site upon UV irradiation. Inactivation of SNF5 affects the recruitment of ATM checkpoint kinase to the damage site and ATM activation by phosphorylation. SNF5 deficiency results in a defect in H2AX and BRCA1 phosphorylation at the damage site. SNF5/INI1 forms a functional complex with p300/GATA4 on hypertrophic response gene promoters in cardiomyocytes. It plays an inhibitory role in cardiomyocyte hypertrophy by suppressing p300/GATA4-dependent transcription. SNF5 genetic mutations were first reported in malignant rhabdoid tumors.

Antibodies; Cancer Marker

Western Blot (WB)

WB: 1-2 ug/ml

Fig-1: Expression analysis of INI-1/SNF5. Anti-INI-1/SNF5 antibody (11-7550) was used at 1 ug/ml on MCF-7 lysate.

43,158 Da

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1

BAF47; INI1; SNF5L1; BAF47; hSNF5  [Similar Products]

The protein encoded by this gene is part of a complex that relieves repressive chromatin structures, allowing the transcriptional machinery to access its targets more effectively. The encoded nuclear protein may also bind to and enhance the DNA joining activity of HIV-1 integrase. This gene has been found to be a tumor suppressor, and mutations in it have been associated with malignant rhabdoid tumors. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2015]

SMARCB1: a core component of the BAF (hSWI/SNF) complex. This family of complexes remodels chromatin structures, enabling transcription factors to gain access to DNA. They play important roles in cell proliferation and differentiation, in cellular antiviral activities and inhibition of tumor formation. The BAF complex is able to create a stable, altered form of chromatin that constrains fewer negative supercoils than normal. This change in supercoiling is due to the conversion of up to one-half of the nucleosomes on polynucleosomal arrays into asymmetric structures, termed altosomes, each composed of 2 histones octamers. Stimulates in vitro the remodeling activity of SMARCA4. Involved in activation of CSF1 promoter. Belongs to the neural progenitors- specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their ***** state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain BAF53A and PHF10, are exchanged for homologous alternative BAF53B and BAF45B or BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. Plays a key role in cell-cycle control and causes cell cycle arrest in G0/G1. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand- bound VDR-mediated transrepression of the CYP27B1 gene. Component of the BAF (hSWI/SNF) complex, which includes at least actin (ACTB), ARID1A, ARID1B, SMARCA2, SMARCA4, BAF53A, BAF53B, SMARCE1 SMARCC1, SMARCC2, SMARCB1, and one or more of SMARCD1, SMARCD2, or SMARCD3. In muscle cells, the BAF complex also contains DPF3. Component of the WINAC complex, at least composed of SMARCA2, SMARCA4, SMARCB1, SMARCC1, SMARCC2, SMARCD1, SMARCE1, ACTL6A, WSTF, ARID1A, SUPT16H, CHAF1A and TOP2B. Binds to double-stranded DNA. Interacts with EphB4 and MAEL. Interacts with GADD34. Binds tightly to the human immunodeficiency virus-type 1 (HIV-1) integrase in vitro and stimulates its DNA-joining activity. Interacts with human papillomavirus 18 E1 protein to stimulates its viral replication. Belongs to the SNF5 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Transcription, coactivator/corepressor; Tumor suppressor

Chromosomal Location of Human Ortholog: 22q11.23|22q11

Cellular Component: nuclear chromatin; nucleolus; nucleoplasm; nucleus; protein complex; SWI/SNF complex

Molecular Function: DNA binding; nucleosomal DNA binding; p53 binding; protein binding; Tat protein binding; transcription coactivator activity

Biological Process: ATP-dependent chromatin remodeling; cell differentiation; chromatin remodeling; DNA integration; DNA repair; negative regulation of cell proliferation; nucleosome disassembly; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter

Disease: Mental Retardation, Autosomal Dominant 15; Rhabdoid Tumor Predisposition Syndrome 1; Schwannomatosis 1

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