ACTG1 / Gamma Actin, Monoclonal Antibody

Anti-ACTG1 / Gamma Actin Antibody (N-Terminus, clone 2A3) IHC-plus; ACTG; Actin; gamma 1; BRWS2; Cytoskeletal gamma-actin; DFNA20; Actin; cytoplasmic 2; Gamma-actin; DFNA26; Human ACTG1; Gamma Actin

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG2b

2A3

Mouse

Clone 2A3 recognizes cytoplasmic actin gamma (known also as ACTG and Gamma Actin), a 41.8 kD cytoskeletal protein. Actins are highly conserved proteins that are involved in cell motility, structure and integrity and are ubiquitously expressed in all eukaryotic cells. Six different isoforms of actin have been identified. Clone 2A3 is highly specific for actin gamma and does not react with other actin isoforms.

Purified

PBS, 0.09% sodium azide

1 mg/ml (lot specific)

+4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Small volumes of anti-ACTG1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB)

IF, IHC-P (10 ug/ml), WB (1:100 - 1:1000)

Anti-ACTG1 / Gamma Actin antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

HeLa whole cell lysate stained with Mouse anti-Human Actin Gamma followed by Rabbit F(ab')2 anti-Mouse IgG:DyLight800. Visualized using the LiCOR Odyssey imager

41,793 Da

actin, gamma 1

actin, cytoplasmic 2; cytoskeletal gamma-actin; epididymis luminal protein 176

Actin, cytoplasmic 2

ACTG  [Similar Products]

ACTG_HUMAN

Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in non-muscle cells. Mutations in this gene are associated with DFNA20/26, a subtype of autosomal dominant non-syndromic sensorineural progressive hearing loss. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2011]

ACTG1: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Belongs to the actin family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: filamentous actin; extracellular space; cytoskeleton; myofibril; membrane; plasma membrane; nucleus; cytosol

Molecular Function: identical protein binding; protein binding; structural constituent of cytoskeleton; ubiquitin protein ligase binding; ATP binding

Biological Process: intercellular junction assembly and maintenance; retinal homeostasis; axon guidance; ephrin receptor signaling pathway; innate immune response; sarcomere organization; cell motility; vascular endothelial growth factor receptor signaling pathway

Disease: Baraitser-winter Syndrome 2; Deafness, Autosomal Dominant 20

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