Product Name
TRPC6, siRNA
Full Product Name
TRPC6 siRNA (Mouse)
Product Synonym Names
TRP6; TRRP6; Short transient receptor potential channel 6; TrpC6; Calcium entry channel; Transient receptor protein 6; TRP-6
Product Gene Name
TRPC6 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q61143
Specificity
TRPC6 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse TRPC6 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of TRPC6 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
TRPC6 sirna
siRNA to inhibit TRPC6 expression using RNA interference
Applications Tested/Suitable for TRPC6 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for TRPC6. It may not necessarily be applicable to this product.
NCBI Accession #
NP_038866.2
[Other Products]
NCBI GenBank Nucleotide #
NM_013838.2
[Other Products]
UniProt Primary Accession #
Q61143
[Other Products]
UniProt Secondary Accession #
Q9Z2J1; B9EIW2[Other Products]
UniProt Related Accession #
Q61143[Other Products]
Molecular Weight
106,681 Da
NCBI Official Full Name
short transient receptor potential channel 6 isoform 1
NCBI Official Synonym Full Names
transient receptor potential cation channel, subfamily C, member 6
NCBI Official Symbol
Trpc6 [Similar Products]
NCBI Official Synonym Symbols
TRP-6; Trrp6; mtrp6; AV025995; LLHWJM002; LLHWJM003; LLHWJM004
[Similar Products]
NCBI Protein Information
short transient receptor potential channel 6
UniProt Protein Name
Short transient receptor potential channel 6
UniProt Synonym Protein Names
Calcium entry channel; Transient receptor protein 6; TRP-6
Protein Family
Short transient receptor potential channel
UniProt Gene Name
Trpc6 [Similar Products]
UniProt Synonym Gene Names
Trp6; Trrp6; TrpC6; TRP-6 [Similar Products]
UniProt Entry Name
TRPC6_MOUSE
UniProt Comments for TRPC6
TRPC6: Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C. Seems not to be activated by intracellular calcium store depletion. Interacts with MX1 and RNF24. Expressed primarily in placenta, lung, spleen, ovary and small intestine. Expressed in podocytes and is a component of the glomerular slit diaphragm. Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC6 sub-subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Channel, cation; Membrane protein, integral; Membrane protein, multi-pass
Cellular Component: membrane; integral to plasma membrane; integral to membrane; plasma membrane; cytosol
Molecular Function: store-operated calcium channel activity; protein binding; calcium channel activity; ion channel activity; cation channel activity
Biological Process: positive regulation of ion transmembrane transporter activity; elevation of cytosolic calcium ion concentration; transport; calcium ion transport; manganese ion transport; ion transport; positive regulation of calcium ion transport; transmembrane transport
Research Articles on TRPC6
1. L-expressing mice prevented FSGS development and inhibited tubular damage and podocyte loss induced by puromycin aminonucleoside">Gq activation stimulated calcineurin, resulting in CN-dependent TRPC6 upregulation in murine kidneys. TRPC6 deletion in GqQ>L-expressing mice prevented FSGS development and inhibited tubular damage and podocyte loss induced by puromycin aminonucleoside
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
