Full Product Name
PKD1 (Phospho-Ser205) Polyclonal Antibody
Product Synonym Names
PRKD1; PKD; PKD1; PRKCM; Serine/threonine-protein kinase D1; Protein kinase C mu type; Protein kinase D; nPKC-D1; nPKC-mu
Product Gene Name
anti-PKD1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q15139
Species Reactivity
Human, Mouse, Rat
Specificity
Phospho-PKD1 (S205) Polyclonal Antibody detects endogenous levels of PKD1 protein only when phosphorylated at S205.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration
1 mg/ml (lot specific)
Immunogen Description
Synthesized peptide derived from human PKD1 around the phosphorylation site of S205.
Modification
Phospho-Ser205
Preparation and Storage
Store at -20 degree C/1 year
Other Notes
Small volumes of anti-PKD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-PKD1 antibody
Phospho-specific Ab
Applications Tested/Suitable for anti-PKD1 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-PKD1 antibody
Western blotting: 1:500 - 1:2000
Immunohistochemistry: 1:100 - 1:300
ELISA: 1:40000
Not yet tested in other applications
Western Blot (WB) of anti-PKD1 antibody
Western Blot analysis of 3T3 cells using Phospho-PKD1 (S205) Polyclonal Antibody
NCBI/Uniprot data below describe general gene information for PKD1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_002733.2
[Other Products]
NCBI GenBank Nucleotide #
NM_002742.2
[Other Products]
UniProt Primary Accession #
Q15139
[Other Products]
UniProt Secondary Accession #
A6NL64; B2RAF6[Other Products]
UniProt Related Accession #
Q15139[Other Products]
Molecular Weight
101,704 Da
NCBI Official Full Name
serine/threonine-protein kinase D1
NCBI Official Synonym Full Names
protein kinase D1
NCBI Official Symbol
PRKD1 [Similar Products]
NCBI Official Synonym Symbols
PKD; PKCM; PRKCM; PKC-MU
[Similar Products]
NCBI Protein Information
serine/threonine-protein kinase D1
UniProt Protein Name
Serine/threonine-protein kinase D1
UniProt Synonym Protein Names
Protein kinase C mu type; Protein kinase D; nPKC-D1; nPKC-mu
Protein Family
Polycystin
UniProt Gene Name
PRKD1 [Similar Products]
UniProt Synonym Gene Names
PKD; PKD1; PRKCM [Similar Products]
UniProt Entry Name
KPCD1_HUMAN
NCBI Summary for PKD1
PRKD1 is a serine/threonine kinase that regulates a variety of cellular functions, including membrane receptor signaling, transport at the Golgi, protection from oxidative stress at the mitochondria, gene transcription, and regulation of cell shape, motility, and adhesion (summary by Eiseler et al., 2009 [PubMed 19329994]).[supplied by OMIM, Nov 2010]
UniProt Comments for PKD1
PRKD1: a CAMK kinase of the PKD family. Cleavage by caspase-3 following DNA damage activates it and alters its subcellular localization. Sensitizes cells to apoptosis induced by genotoxic stress. Its cleavage is blocked in cells that over-express the anti-apoptotic Bcl-x(L) protein. Expression of a caspase-resistant mutant partially inhibits DNA damage-induced apoptosis. Its activation by TLR ligands is dependent on MyD88, IRAK4 and -1, but not TRAF6. Essential for MyD88-dependent proinflammatory immune responses. Activated by diacylglycerol and phorbol esters. Binds to the trans-Golgi network and regulates the fission of transport carriers specifically destined to the cell surface. Colocalizes with F-actin at peripheral F-actin-rich structures in membrane ruffles at the edge of lamellipodia in cervical carcinoma cells. Substrates reportedly include critical regulatory proteins including CREB, SSH1L, CTNNB1, HDACs 5 and 7, PKD1, HPK1, MARK2, PIP5K2A, PPP1R14A
Protein type: Protein kinase, CAMK; Autophagy; EC 2.7.11.13; Motility/polarity/chemotaxis; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; CAMK group; PKD family
Chromosomal Location of Human Ortholog: 14q11
Cellular Component: integral to plasma membrane; cytoplasm; plasma membrane; trans-Golgi network; cell cortex; intercellular junction; cytosol; nucleus
Molecular Function: protein serine/threonine kinase activity; identical protein binding; protein binding; protein kinase C activity; metal ion binding; ATP binding
Biological Process: integrin-mediated signaling pathway; sphingolipid metabolic process; positive regulation of I-kappaB kinase/NF-kappaB cascade; sphingolipid biosynthetic process; apoptosis; negative regulation of endocytosis; protein amino acid autophosphorylation; positive regulation of blood vessel endothelial cell migration; activation of CREB transcription factor; signal transduction; positive regulation of peptidyl-serine phosphorylation; Golgi vesicle transport; activation of NF-kappaB transcription factor; cell proliferation; positive regulation of osteoblast differentiation; positive regulation of angiogenesis; peptidyl-serine phosphorylation; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; angiogenesis; vascular endothelial growth factor receptor signaling pathway; inflammatory response; Golgi organization and biogenesis
Research Articles on PKD1
1. a positive relationship between L1 and pPKD1 in both cultured cerebellar neurons and human cerebellar tissue, suggesting that L1 functions in the modulation of PKD1 phosphorylation.
Precautions
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Disclaimer
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