Product Name
Cyclooxygenase 1 (COX-1), Polyclonal Antibody
Full Product Name
Cyclooxygenase 1 Antibody: FITC
Product Synonym Names
Rabbit Anti-Human Cyclooxygenase 1 Polyclonal; EC:1.14.99.1 Antibody; PGHS1 Antibody; Prostaglandin G/H synthase 1 Antibody; PGHS-1 Antibody; Prostaglandin H2 synthase 1 Antibody; Prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase) Antibody; PHS 1 Antibody; EC 1.14.99.1 Antibody; Prostaglandin-endoperoxide synthase 1 Antibody; COX 3 Antibody; PTGS1 Antibody; PGH1_HUMAN Antibody; COX-1 Antibody; PGG/HS Antibody; PTGHS Antibody; Cox3 Antibody; PGH synthase 1 Antibody; Cyclooxygenase-1 Antibody; PHS1 Antibody; Cyclooxygenase 1 Antibody; Cyclooxygenase 3; included Antibody; Partial COX1 proteins; included Antibody; COX 1 Antibody; COX1 Antibody; PCOX1 Antibody
Product Gene Name
anti-COX-1 antibody
[Similar Products]
Matching Pairs
Unconjugated Antibody: Cyclooxygenase 1 (MBS8000265)
FITC Conjugated Antibody: Cyclooxygenase 1 (MBS8000277)
Matching Pairs
Unconjugated Antibody: N/A (MBS8000277)
FITC Conjugated Antibody: Cyclooxygenase 1 (MBS8000277)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P23219
Species Reactivity
Human; Mouse; Rat
Specificity
Detects ~75 kDa.
Purity/Purification
Peptide Affinity Purified
Concentration
1 mg/ml (lot specific)
Immunogen
Synthetic peptide of Human Cyclooxygenase
Cellular Localization
Microsome Membrane; Peripheral Membrane Protein; Endoplasmic Reticulum Membrane; Peripheral Membrane Protein
Storage Buffer
PBS, 50% glycerol, 0.09% sodium azide
Preparation and Storage
Store at -20 degree C.
Other Notes
Small volumes of anti-COX-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-COX-1 antibody
Antibodies; Cardiovascular System; Blood; Platelets; Immunology; Innate Immunity; Macrophage; Cardiovascular System; Atherosclerosis; Cell Signaling; Metabolism; Lipid and lipoprotein Metabolism; Cell Signaling; Metabolism; Redox Metabolism; Metabolism an
Applications Tested/Suitable for anti-COX-1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-COX-1 antibody
WB: 1:1000
ICC/IF: 1:100
Immunocytochemistry/Immunofluorescence (ICC/IF) of anti-COX-1 antibody
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Microsome Membrane, Endoplasmic Reticulum Membrane, Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Cyclooxygenase 1 Antibody. (D) Composite.
Western Blot (WB) of anti-COX-1 antibody
Western blot analysis of Human HeLa and HEK293Trap cell lysates showing detection of 68.7 kDa Cyclooxygenase 1 protein using Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 68.7 kDa.
Western Blot (WB) of anti-COX-1 antibody
Western blot analysis of Mouse Kidney cell lysates showing detection of 68.7 kDa Cyclooxygenase 1 protein using Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Kidney cell lysates. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 68.7 kDa.
Western Blot (WB) of anti-COX-1 antibody
Western blot analysis of Rat Brain cell lysates showing detection of 68.7 kDa Cyclooxygenase 1 protein using Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 68.7 kDa.
NCBI/Uniprot data below describe general gene information for COX-1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000953.2
[Other Products]
NCBI GenBank Nucleotide #
NM_000962.3
[Other Products]
UniProt Primary Accession #
P23219
[Other Products]
UniProt Secondary Accession #
Q15122; Q3HY28; Q3HY29; Q5T7T6; Q5T7T7; Q5T7T8; A8K1V7; B4DHQ2; B4E2S5[Other Products]
UniProt Related Accession #
P23219[Other Products]
Molecular Weight
72,010 Da
NCBI Official Full Name
prostaglandin G/H synthase 1 isoform 1
NCBI Official Synonym Full Names
prostaglandin-endoperoxide synthase 1
NCBI Official Symbol
PTGS1 [Similar Products]
NCBI Official Synonym Symbols
COX1; COX3; PHS1; PCOX1; PES-1; PGHS1; PTGHS; PGG/HS; PGHS-1
[Similar Products]
NCBI Protein Information
prostaglandin G/H synthase 1
UniProt Protein Name
Prostaglandin G/H synthase 1
UniProt Synonym Protein Names
Cyclooxygenase-1; COX-1; Prostaglandin H2 synthase 1; PGH synthase 1; PGHS-1; PHS 1; Prostaglandin-endoperoxide synthase 1
UniProt Gene Name
PTGS1 [Similar Products]
UniProt Synonym Gene Names
COX1; COX-1; PGH synthase 1; PGHS-1; PHS 1 [Similar Products]
NCBI Summary for COX-1
This is one of two genes encoding similar enzymes that catalyze the conversion of arachinodate to prostaglandin. The encoded protein regulates angiogenesis in endothelial cells, and is inhibited by nonsteroidal anti-inflammatory drugs such as aspirin. Based on its ability to function as both a cyclooxygenase and as a peroxidase, the encoded protein has been identified as a moonlighting protein. The protein may promote cell proliferation during tumor progression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
UniProt Comments for COX-1
Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Involved in the constitutive production of prostanoids in particular in the stomach and platelets. In gastric epithelial cells, it is a key step in the generation of prostaglandins, such as prostaglandin E2 (PGE2), which plays an important role in cytoprotection. In platelets, it is involved in the generation of thromboxane A2 (TXA2), which promotes platelet activation and aggregation, vasoconstriction and proliferation of vascular smooth muscle cells.
Research Articles on COX-1
1. Results suggested that in Chinese Han patients with stroke taking aspirin for secondary prevention, PTGS1 polymorphisms may increase the risk of poor functional outcomes and this effect may be modulated by the smoking status. PTGS1 gene-smoking interaction might in part reflect the heterogeneity in the prognosis of patients treated with aspirin.
Precautions
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Disclaimer
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