DPAGT1, Polyclonal Antibody

UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase; GlcNAc-1-P transferase; G1PT; GPT; N-acetylglucosamine-1-phosphate transferase; DPAGT1; DPAGT2

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This DPAGT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-57 amino acids from the N-terminal region of human DPAGT1.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.45 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-DPAGT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

The protein encoded by this gene is an enzyme that
catalyzes the first step in the dolichol-linked oligosaccharide
pathway for glycoprotein biosynthesis. This enzyme belongs to the
glycosyltransferase family 4. This protein is an integral membrane
protein of the endoplasmic reticulum. The congenital disorder of
glycosylation type Ij is caused by mutation in the gene encoding
this enzyme.

Western Blot (WB), ELISA (EIA)

WB~~1:1000

DPAGT1 Antibody (N-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the DPAGT1 antibody detected the DPAGT1 protein (arrow).

DPAGT1 Antibody (N-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the DPAGT1 antibody detected the DPAGT1 protein (arrow).

46090

dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase)

UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase

UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase

DPAGT2; G1PT; GPT  [Similar Products]

GPT_HUMAN

The protein encoded by this gene is an enzyme that catalyzes the first step in the dolichol-linked oligosaccharide pathway for glycoprotein biosynthesis. This enzyme belongs to the glycosyltransferase family 4. This protein is an integral membrane protein of the endoplasmic reticulum. The congenital disorder of glycosylation type Ij is caused by mutation in the gene encoding this enzyme. [provided by RefSeq, Jul 2008]

DPAGT1: Catalyzes the initial step in the synthesis of dolichol- P-P-oligosaccharides. Defects in DPAGT1 are the cause of congenital disorder of glycosylation type 1J (CDG1J). CDGs are a family of severe inherited diseases caused by a defect in protein N- glycosylation. They are characterized by under-glycosylated serum proteins. These multisystem disorders present with a wide variety of clinical features, such as disorders of the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions. Belongs to the glycosyltransferase 4 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Glycan Metabolism - N-glycan biosynthesis; Endoplasmic reticulum; Transferase; Membrane protein, multi-pass; Membrane protein, integral; EC 2.7.8.15

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: endoplasmic reticulum membrane; membrane; intracellular membrane-bound organelle; integral to membrane; integral to endoplasmic reticulum membrane

Molecular Function: UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity; transferase activity, transferring glycosyl groups; phospho-N-acetylmuramoyl-pentapeptide-transferase activity

Biological Process: UDP-N-acetylglucosamine metabolic process; polysaccharide biosynthetic process; cellular protein metabolic process; dolichol-linked oligosaccharide biosynthetic process; protein amino acid N-linked glycosylation; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification; dolichol biosynthetic process; protein oligomerization

Disease: Congenital Disorder Of Glycosylation, Type Ij; Myasthenic Syndrome, Congenital, With Tubular Aggregates 2

Sengupta, P.K., et al. J. Biol. Chem. 285(41):31164-31173(2010)
Nita-Lazar, M., et al. Cancer Res. 69(14):5673-5680(2009)
Bretthauer, R.K. Curr Drug Targets 10(6):477-482(2009)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Wu, X., et al. Hum. Mutat. 22(2):144-150(2003)

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