Full Product Name
QDPR siRNA (Mouse)
Product Synonym Names
DHPR; Dihydropteridine reductase; HDHPR; Quinoid dihydropteridine reductase
Product Gene Name
QDPR sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8BVI4
Specificity
QDPR siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse QDPR gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of QDPR sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
QDPR sirna
siRNA to inhibit QDPR expression using RNA interference
Applications Tested/Suitable for QDPR sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for QDPR. It may not necessarily be applicable to this product.
NCBI Accession #
NP_077198.1
[Other Products]
NCBI GenBank Nucleotide #
NM_024236.2
[Other Products]
UniProt Primary Accession #
Q8BVI4
[Other Products]
UniProt Secondary Accession #
Q3TT09; Q9D0K4[Other Products]
UniProt Related Accession #
Q8BVI4[Other Products]
Molecular Weight
25,570 Da
NCBI Official Full Name
dihydropteridine reductase
NCBI Official Synonym Full Names
quinoid dihydropteridine reductase
NCBI Official Symbol
Qdpr [Similar Products]
NCBI Official Synonym Symbols
Dhpr; PKU2; D5Ertd371e; 2610008L04Rik
[Similar Products]
NCBI Protein Information
dihydropteridine reductase
UniProt Protein Name
Dihydropteridine reductase
UniProt Synonym Protein Names
HDHPR; Quinoid dihydropteridine reductase
Protein Family
Dihydropteridine reductase
UniProt Gene Name
Qdpr [Similar Products]
UniProt Synonym Gene Names
Dhpr [Similar Products]
UniProt Entry Name
DHPR_MOUSE
UniProt Comments for QDPR
QDPR: The product of this enzyme, tetrahydrobiopterin (BH-4), is an essential cofactor for phenylalanine, tyrosine, and tryptophan hydroxylases. Defects in QDPR are the cause of BH4-deficient hyperphenylalaninemia type C (HPABH4C); also called dihydropteridine reductase deficiency (DHPR deficiency) or hyperphenylalaninemia tetrahydrobiopterin-deficient due to DHPR deficiency or quinoid dihydropteridine reductase deficiency (QDPR deficiency). HPABH4C is a rare autosomal recessive disorder characterized by hyperphenylalaninemia and severe neurologic symptoms (malignant hyperphenylalaninemia) including axial hypotonia and truncal hypertonia, abnormal thermogenesis, and microcephaly. These signs are attributable to depletion of the neurotransmitters dopamine and serotonin, whose syntheses are controlled by tryptophan and tyrosine hydroxylases that use BH-4 as cofactor. These patients do not respond to phenylalanine- restricted diet. HPABH4C is lethal if untreated. Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Protein type: Oxidoreductase; EC 1.5.1.34; Cofactor and Vitamin Metabolism - folate biosynthesis
Cellular Component: neuron projection; mitochondrion; cytoplasm; cytosol
Molecular Function: protein homodimerization activity; 6,7-dihydropteridine reductase activity; oxidoreductase activity
Biological Process: tetrahydrobiopterin biosynthetic process; metabolic process
Research Articles on QDPR
1. wide alterations in folate-associated metabolism in the Qdpr(-/-) mice
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
