DAX1/NR0B1, Polyclonal Antibody

NR0B1; DAX1; nuclear receptor subfamily 0, group B, member 1; AHC; AHCH; AHX; DAX-1; DSS; GTD; HHG; NROB1; dosage-sensitive sex reversal; gonadotropin deficiency; nuclear hormone receptor; NR0B1 antibody; DAX1 antibody; nuclear receptor subfamily 0; group B; member 1 antibody; AHC antibody; AHCH antibody; AHX antibody; DAX-1 antibody; DSS antibody; GTD antibody; HHG antibody; NROB1 antibody; dosage-sensitive sex reversal antibody; gonadotropin deficiency antibody; nuclear hormone receptor antibody; DAX1 / NR0B1

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Goat

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul (lot specific)

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Manufactured in an ISO 9001:2008 Certified Laboratory.

Small volumes of anti-NR0B1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Peptide ELISA (EIA), Western Blot (WB)

Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 50-55kDa band observed in human ovary and human testis lysates (calculated MW of 51.7kDa according to NP_000466.2). Recommended concentration: 0.5-1.5ug/ml.

(0.5ug/ml) staining of human testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

43,590 Da

nuclear receptor subfamily 0 group B member 1

nuclear receptor subfamily 0 group B member 1

Nuclear receptor subfamily 0 group B member 1

AHC; DAX1  [Similar Products]

NR0B1_HUMAN

This gene encodes a protein that contains a DNA-binding domain. The encoded protein acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in this gene result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism. [provided by RefSeq, Jul 2008]

NR0B1: orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency. Homodimerizes with STF-1, NR5A2, NR0B2 and with COPS2. Shuttles between the cytoplasm and nucleus. Homodimers exits in the cytoplasm and in the nucleus. Homodimerization involved an interaction between amino and carboxy termini involving LXXLL motifs and steroid binding domain (AF-2 motif). Heterodimerizes with STF-1 and NROB2 through its N-terminal LXXLL motifs. Defects in NR0B1 are the cause of congenital X-linked adrenal hypoplasia. Two alternatively spliced human isoforms have been described.

Protein type: Nuclear receptor

Chromosomal Location of Human Ortholog: Xp21.3

Cellular Component: cytoplasm; membrane; nucleoplasm; nucleus; polysomal ribosome

Molecular Function: AF-2 domain binding; DNA binding; DNA hairpin binding; ligand-dependent nuclear receptor activity; protein binding; protein domain specific binding; protein homodimerization activity; RNA binding; sequence-specific DNA binding; steroid hormone receptor activity; steroid hormone receptor binding; transcription corepressor activity; transcription factor binding

Biological Process: adrenal gland development; gonad development; hypothalamus development; intracellular receptor-mediated signaling pathway; Leydig cell differentiation; male gonad development; male sex determination; negative regulation of cell differentiation; negative regulation of steroid hormone receptor signaling pathway; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; pituitary gland development; protein localization; Sertoli cell differentiation; spermatogenesis; steroid biosynthetic process; steroid hormone mediated signaling; transcription initiation from RNA polymerase II promoter

Disease: 46,xy Sex Reversal 2; Adrenal Hypoplasia, Congenital

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