Product Name
Cyclooxygenase 1 (COX1), Polyclonal Antibody
Full Product Name
Cyclooxygenase 1 Antibody: APC/Cy7
Product Synonym Names
Rabbit Anti-Human Cyclooxygenase 1 Polyclonal; EC:1.14.99.1 Antibody; PGHS1 Antibody; Prostaglandin G/H synthase 1 Antibody; PGHS-1 Antibody; Prostaglandin H2 synthase 1 Antibody; Prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase) Antibody; PHS 1 Antibody; EC 1.14.99.1 Antibody; Prostaglandin-endoperoxide synthase 1 Antibody; COX 3 Antibody; PTGS1 Antibody; PGH1_HUMAN Antibody; COX-1 Antibody; PGG/HS Antibody; PTGHS Antibody; Cox3 Antibody; PGH synthase 1 Antibody; Cyclooxygenase-1 Antibody; PHS1 Antibody; Cyclooxygenase 1 Antibody; Cyclooxygenase 3; included Antibody; Partial COX1 proteins; included Antibody; COX 1 Antibody; COX1 Antibody; PCOX1 Antibody
Product Gene Name
anti-COX1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P23219
Species Reactivity
Human, Mouse, Rat
Specificity
Detects ~75 kDa.
Purity/Purification
Peptide Affinity Purified
Form/Format
PBS, 50% Glycerol, 0.09% Sodium Azide
Concentration
1 mg/ml (lot specific)
Immunogen
Synthetic peptide of Human Cyclooxygenase
Cellular Localization
Microsome Membrane; Peripheral Membrane Protein; Endoplasmic Reticulum Membrane; Peripheral Membrane Protein
Certificate of Analysis
A 1:1000 dilution was sufficient for detection of Cyclooxygenase in 15 ug of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Product Notes
Conjugated primary antibodies can be good options or alternatives to secondary antibody's for detecting a target protein. The lab manufactures a conjugated antibody from a primary parent antibody by attaching (conjugating) an available tag such as a fluorophore or protein, allowing detection of the target protein without using a secondary antibody. Conjugated primary antibodies may potentially be used to replace primary/secondary antibody pairs in various applications, such as Enzyme-Linked Immunosorbent Assay (ELISA), Flow Cytometry, Fluorescence-activated cell sorting, Förster Resonance Energy Transfer (FRET). Immunocytochemistry, Immunofluorescence, Immunohistochemistry, or Western Blot. The suitability of applications for conjugates are user determined and we cannot guarantee that any antibody-conjugate combination has been tested for use in a particular application.
Please note: Conjugate tags may potentially bind in the antibody paratope, which can limit binding of the antibody to the antigen. Binding in the antibody paratope may affect the ability of the antibody to bind to the corresponding antigen (protein target) in various species and applications. There is no way to confirm or guarantee the location of the antibody tag as we cannot control the binding of the conjugate to the antibody. Researchers are responsible for evaluating antibody conjugates for the suitability of their applications.
Preparation and Storage
Store at-20 degree C.
Other Notes
Small volumes of anti-COX1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-COX1 antibody
Cardiovascular System; Blood; Platelets; Immunology; Innate Immunity; Macrophage; Cardiovascular System; Atherosclerosis; Cell Signaling; Metabolism; Lipid and Lipoprotein Metabolism; Cell Signaling; Metabolism; Redox Metabolism; Metabolism and Oxidative
Applications Tested/Suitable for anti-COX1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-COX1 antibody
WB: 1:1000
ICC/IF: 1:100
Immunocytochemistry (ICC) of anti-COX1 antibody
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Kidney cell lysates. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 68.7 kDa.
NCBI/Uniprot data below describe general gene information for COX1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000953.2
[Other Products]
NCBI GenBank Nucleotide #
NP_000953.2
[Other Products]
UniProt Primary Accession #
P23219
[Other Products]
UniProt Related Accession #
P23219[Other Products]
NCBI Official Full Name
prostaglandin G/H synthase 1 isoform 1
NCBI Official Synonym Full Names
prostaglandin-endoperoxide synthase 1
NCBI Official Symbol
PTGS1 [Similar Products]
NCBI Official Synonym Symbols
COX1; COX3; PHS1; PCOX1; PES-1; PGHS1; PTGHS; PGG/HS; PGHS-1
[Similar Products]
NCBI Protein Information
prostaglandin G/H synthase 1
UniProt Protein Name
Prostaglandin G/H synthase 1
UniProt Synonym Protein Names
Cyclooxygenase-1; COX-1; Prostaglandin H2 synthase 1; PGH synthase 1; PGHS-1; PHS 1; Prostaglandin-endoperoxide synthase 1
UniProt Gene Name
PTGS1 [Similar Products]
UniProt Synonym Gene Names
COX1; COX-1; PGH synthase 1; PGHS-1; PHS 1 [Similar Products]
UniProt Entry Name
PGH1_HUMAN
NCBI Summary for COX1
This is one of two genes encoding similar enzymes that catalyze the conversion of arachinodate to prostaglandin. The encoded protein regulates angiogenesis in endothelial cells, and is inhibited by nonsteroidal anti-inflammatory drugs such as aspirin. Based on its ability to function as both a cyclooxygenase and as a peroxidase, the encoded protein has been identified as a moonlighting protein. The protein may promote cell proliferation during tumor progression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
UniProt Comments for COX1
COX-1: May play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells. Homodimer. Belongs to the prostaglandin G/H synthase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 1.14.99.1; Lipid Metabolism - arachidonic acid; Oxidoreductase
Chromosomal Location of Human Ortholog: 9q32-q33.3
Cellular Component: endoplasmic reticulum membrane; photoreceptor outer segment; intracellular membrane-bound organelle; cytoplasm; nucleus
Molecular Function: prostaglandin-endoperoxide synthase activity; peroxidase activity; dioxygenase activity; metal ion binding; heme binding
Biological Process: xenobiotic metabolic process; regulation of blood pressure; cyclooxygenase pathway; arachidonic acid metabolic process; lipid metabolic process; response to oxidative stress; inflammatory response; prostaglandin biosynthetic process; regulation of cell proliferation
Research Articles on COX1
1. COX1 inhibition significantly triggered cell death.
Precautions
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Disclaimer
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