IDH2, Polyclonal Antibody

IDH2; isocitrate dehydrogenase 2 (NADP+), mitochondrial; ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH; NADP+-specific ICDH; isocitrate dehydrogenase, mitochondrial; oxalosuccinate decarboxylase; ICD-M antibody; IDH antibody; IDHM antibody; IDP antibody; IDPM antibody; isocitrate dehydrogenase 2 (NADP+); mitochondrial antibody; isocitrate dehydrogenase; mitochondrial antibody; mNADP-IDH antibody; NADP+-specific ICDH antibody; oxalosuccinate decarboxylase antibody; IDH2 antibody

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Goat

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul (lot specific)

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Manufactured in an ISO 9001:2008 Certified Laboratory.

Small volumes of anti-IDH2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Peptide ELISA (EIA), Western Blot (WB)

Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 48kDa band observed in Mouse, Rat and Pig Heart lysates (calculated MW of 50.9kDa according to Mouse NP_766599.2). Recommended concentration: 0.1-0.3ug/ml.

(0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

(0.1ug/ml) staining of Mouse (A), Rat (B) and Pig (C) Heart lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

45,180 Da

isocitrate dehydrogenase (NADP(+)) 2, mitochondrial

isocitrate dehydrogenase [NADP], mitochondrial

Isocitrate dehydrogenase [NADP], mitochondrial

IDH  [Similar Products]

IDHP_HUMAN

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

IDH2: a mitochondrial oxidoreductase that catalyzes the third step of the citric acid cycle: the oxidative decarboxylation of isocitrate, consuming NADP(+), and producing alpha-ketoglutarate (alpha-KG) and CO2. Can also use oxalosuccinate as a substrate. Alpha-KG is an activator the dioxygenases that hydroxylate the transcription factor HIF and lead to its degradation by VHL. Since HIF turns on oncogenic pathways, IDH2 has apparent tumor suppressor activity. Homo-dimerization is required for activity. Each subunit binds 1 magnesium or manganese ion. May tightly associate or interact with the pyruvate dehydrogenase complex. A somatic mutation resulting in the R172K conversion results in a neomorphic enzyme activity that converts isocitrate to R(-)-2-hydroxyglutarate (2HG) occurs in a significant percentage of patients with cytogenetically normal acute myeloid leukemia (AML). Elevated levels of 2HG are also correlated with an elevated risk of malignant brain tumors.

Protein type: Oxidoreductase; Other Amino Acids Metabolism - glutathione; Mitochondrial; EC 1.1.1.42; Carbohydrate Metabolism - citrate (TCA) cycle

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: cytosol; mitochondrial inner membrane; mitochondrial matrix; mitochondrion; peroxisome

Molecular Function: isocitrate dehydrogenase (NADP+) activity; magnesium ion binding; NAD binding

Biological Process: 2-oxoglutarate metabolic process; carbohydrate metabolic process; glyoxylate cycle; isocitrate metabolic process; tricarboxylic acid cycle

Disease: D-2-hydroxyglutaric Aciduria 2

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